FastQCFastQC Report
Thu 26 May 2016
ERR580181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8145194
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197510.24248655096490027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146940.18040085969714165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88580.10875124644053906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130050.048.7750171
GTATCAA242400.042.385241
CAACGCA270550.036.2592285
ATCAACG276200.035.5159763
TATCAAC302950.033.7039342
GTACTAG69600.032.1583331
AACGCAG313800.032.1322866
GTGGTAT36800.031.1772521
ACGCAGA328300.029.7989797
GCATCGT70850.029.7772588
TCAACGC335800.029.6197434
CGCAGAG333000.029.3069388
TAGGCAT88900.028.8163135
GTACATG368800.027.6289141
GGGCACC74050.027.54898894
CTAGGCA96200.027.2161354
TACATGG373900.026.830622
GCAGAGT359600.026.6851889
ACATGGG370650.026.3388883
CTGTGCG91750.025.6605459
Produced by FastQC (version 0.11.5)