FastQCFastQC Report
Thu 26 May 2016
ERR580181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8145194
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177250.2176129874868542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142320.17472880326730092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86600.10632036511346445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136350.047.892941
GTATCAA242350.040.4083061
CAACGCA275900.033.763575
ATCAACG281950.033.105763
GTACTAG69900.031.947791
TATCAAC309000.031.5096342
AACGCAG319100.030.1043436
GGGCACC75150.028.32985194
TCAACGC335950.028.203934
ACGCAGA334950.027.6695467
GTGGTAT42350.027.6420571
CGCAGAG341550.026.942068
TAGGCAT92150.026.8279085
GTACATG375400.026.4749491
CTGTGCG89650.026.1581299
TAGTACT91400.026.1222924
TACATGG378100.025.8629722
ACATGGG372950.025.443813
CTAGGCA102200.024.741454
GCAGAGT370000.024.5519839
Produced by FastQC (version 0.11.5)