FastQCFastQC Report
Thu 26 May 2016
ERR580180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3540885
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114390.3230548295129608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98280.27755772921176486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58900.16634259514217492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.054.6453741
GTATCAA99000.038.947011
CAACGCA118700.031.4016285
ATCAACG119350.031.2292863
TATCAAC126200.030.2473812
AACGCAG127450.029.6749086
TCAACGC127550.029.297374
GTGGTAT20850.028.641331
GTACATG114000.027.38791
TACATGG115700.026.4914282
ACATGGG112500.026.1954773
ACGCAGA143950.025.8821877
GTACTAG17450.025.599041
CGCAGAG147700.025.0985288
TGGTATC23750.024.148342
CATGGGG85900.023.8033524
GCATCGT16550.022.9952838
GCAGAGT162250.022.443649
TCTAACG7400.022.2345032
CTAACGC7000.022.1578273
Produced by FastQC (version 0.11.5)