FastQCFastQC Report
Thu 26 May 2016
ERR580180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3540885
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101100.2855218398790133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91390.25809931697866495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60330.17038113353017678No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.055.8212471
GTATCAA95400.041.0472681
CAACGCA120000.031.8819545
ATCAACG120600.031.7242343
TATCAAC126600.030.5608942
GTGGTAT19450.030.2119031
TCAACGC128300.030.0034564
AACGCAG129950.029.8008176
ACGCAGA145150.026.2591727
CGCAGAG150600.025.2156288
GTACATG109550.025.103321
TACATGG111900.024.2407262
TGGTATC22850.024.0713022
ACATGGG109600.023.9726093
GTACTAG15150.023.5824341
CTGTGCG19300.022.8888119
GCAGAGT164800.022.4139339
CTAGTAC21100.022.2757113
CATGGGG84850.021.6036324
TAACGCC6650.021.2037984
Produced by FastQC (version 0.11.5)