##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580180_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3540885
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.265322934803024	33.0	31.0	34.0	30.0	34.0
2	32.41942311032411	34.0	31.0	34.0	31.0	34.0
3	32.468459438812616	34.0	31.0	34.0	31.0	34.0
4	36.07483807014348	37.0	35.0	37.0	35.0	37.0
5	35.99996780465901	37.0	35.0	37.0	35.0	37.0
6	36.034601519111746	37.0	35.0	37.0	35.0	37.0
7	35.97282402563201	37.0	35.0	37.0	35.0	37.0
8	35.96226847242991	37.0	35.0	37.0	35.0	37.0
9	37.64842941806921	39.0	37.0	39.0	35.0	39.0
10-11	37.57754149033363	39.0	37.0	39.0	35.0	39.0
12-13	37.54380599765312	39.0	37.0	39.0	35.0	39.0
14-15	38.847407074784975	40.0	38.0	41.0	35.0	41.0
16-17	38.8297562614996	40.0	38.0	41.0	35.0	41.0
18-19	38.81794170101543	40.0	38.0	41.0	35.0	41.0
20-21	38.78574071171472	40.0	38.0	41.0	35.0	41.0
22-23	38.69599831680498	40.0	38.0	41.0	34.0	41.0
24-25	38.61999683694896	40.0	38.0	41.0	34.0	41.0
26-27	38.48223763268223	40.0	38.0	41.0	34.0	41.0
28-29	38.359335731038996	40.0	38.0	41.0	34.0	41.0
30-31	38.18133249173582	40.0	38.0	41.0	34.0	41.0
32-33	38.05863887135561	40.0	37.0	41.0	33.5	41.0
34-35	37.95106336410248	40.0	37.0	41.0	33.0	41.0
36-37	37.81227856877588	40.0	37.0	41.0	33.0	41.0
38-39	37.68234650376954	40.0	37.0	41.0	33.0	41.0
40-41	37.55837820205966	40.0	37.0	41.0	32.5	41.0
42-43	37.555187050694954	40.0	37.0	41.0	33.0	41.0
44-45	37.51859182097131	40.0	37.0	41.0	32.5	41.0
46-47	37.33000605780758	40.0	36.5	41.0	32.0	41.0
48-49	37.13320681129153	40.0	36.0	41.0	31.0	41.0
50-51	36.94584856610706	39.0	36.0	41.0	31.0	41.0
52-53	36.78188884982144	39.0	35.5	41.0	31.0	41.0
54-55	36.56744175538036	39.0	35.0	40.5	30.0	41.0
56-57	36.33765329853977	39.0	35.0	40.0	30.0	41.0
58-59	36.10624646098363	38.0	35.0	40.0	29.5	41.0
60-61	35.85269628920453	38.0	35.0	40.0	29.0	41.0
62-63	35.5531919280067	37.5	34.0	40.0	28.5	41.0
64-65	35.25378740060748	37.0	34.0	40.0	28.0	41.0
66-67	34.930458204657874	36.5	34.0	39.0	28.0	41.0
68-69	34.5987723408131	36.0	34.0	39.0	28.0	40.5
70-71	34.254138725205706	35.5	33.5	39.0	27.0	40.0
72-73	33.920845212425704	35.0	33.0	38.0	26.0	40.0
74-75	33.55000713098562	35.0	33.0	37.0	26.0	39.5
76-77	32.037060791299346	34.0	30.5	35.5	25.0	38.5
78-79	32.97226823237693	35.0	32.5	36.0	26.0	39.0
80-81	32.96242874309671	35.0	33.0	36.0	26.0	38.5
82-83	32.718684170765215	35.0	33.0	36.0	26.0	37.0
84-85	32.47971340498209	35.0	32.5	35.0	26.0	37.0
86-87	32.253422378868564	35.0	32.0	35.0	25.0	36.5
88-89	32.04342191288336	34.5	32.0	35.0	25.0	36.0
90-91	31.85230161386207	34.0	32.0	35.0	25.0	36.0
92-93	31.674487027960524	34.0	32.0	35.0	24.5	35.5
94-95	31.51363275000459	34.0	31.0	35.0	24.0	35.0
96-97	31.363626325057155	34.0	31.0	35.0	24.0	35.0
98-99	31.227755490505906	34.0	31.0	35.0	23.0	35.0
100	29.232806770058897	33.0	27.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08749686609571938
1101	2	-0.0938645262007256
1101	3	-0.05600311637277855
1101	4	-0.061747536733093966
1101	5	-0.024441385265689064
1101	6	-0.03521864117335838
1101	7	-0.07276567863720373
1101	8	-0.07721133637988942
1101	9	-0.10845128011300886
1101	10-11	-0.07758737840472918
1101	12-13	-0.0877567163201789
1101	14-15	-0.09288018207504223
1101	16-17	-0.08378038620274708
1101	18-19	-0.08824730660358426
1101	20-21	-0.07710108311258779
1101	22-23	-0.16096611256604376
1101	24-25	-0.09461924700865154
1101	26-27	-0.23984109647977192
1101	28-29	-0.20267046615979467
1101	30-31	-0.12375277809383078
1101	32-33	-0.16266370669210772
1101	34-35	-0.22434467094888078
1101	36-37	-0.20188579080546987
1101	38-39	-0.18389673861007338
1101	40-41	-0.14474877101774553
1101	42-43	-0.28042000273844536
1101	44-45	-0.2249382469233936
1101	46-47	-0.23810778359986529
1101	48-49	-0.08836246503702228
1101	50-51	-0.10207266797606707
1101	52-53	-0.16526429245921292
1101	54-55	-0.10669775265289871
1101	56-57	-0.08515848785706481
1101	58-59	-0.1797061615577462
1101	60-61	-0.20299600953929087
1101	62-63	-0.17076082591880493
1101	64-65	-0.264790769958978
1101	66-67	-0.20700303463227243
1101	68-69	-0.2605914094675228
1101	70-71	-0.19710773692585093
1101	72-73	-0.25602575570804476
1101	74-75	-0.2931417043522728
1101	76-77	-0.2191637617646336
1101	78-79	-0.1472825083328857
1101	80-81	-0.07416652446153194
1101	82-83	-0.1146167165075127
1101	84-85	-0.17037704446008917
1101	86-87	-0.23075258854752434
1101	88-89	-0.24740214149523965
1101	90-91	-0.26625452984464104
1101	92-93	-0.25446964460639165
1101	94-95	-0.3155955851268111
1101	96-97	-0.40925500904632983
1101	98-99	-0.31118280010187505
1101	100	-0.26084745138320997
1102	1	-0.04123951375736823
1102	2	-0.07256273682947523
1102	3	-9.142444531633487E-4
1102	4	-0.004487313210852051
1102	5	-0.0283895314011815
1102	6	0.013932387220414455
1102	7	-0.007088323474462754
1102	8	0.021275035905652828
1102	9	-0.02966915313620433
1102	10-11	-0.08909456323230813
1102	12-13	-0.06352819944183352
1102	14-15	-0.04333979041104641
1102	16-17	0.022353319367191204
1102	18-19	-0.0371052501766016
1102	20-21	-0.08595912970311304
1102	22-23	-0.02488873611301301
1102	24-25	-0.055686911915003634
1102	26-27	-0.024055293923993304
1102	28-29	-0.061115777382134695
1102	30-31	-0.05994793790188879
1102	32-33	-0.1096538569302723
1102	34-35	-0.13620245870790626
1102	36-37	-0.20880633554489947
1102	38-39	-0.17479866121731646
1102	40-41	-0.12429065214326585
1102	42-43	-0.02695764855651106
1102	44-45	-0.08600745709241409
1102	46-47	0.019290569235970167
1102	48-49	0.0033966491154018286
1102	50-51	0.0141605310481836
1102	52-53	-0.05619046710566522
1102	54-55	-0.11523292519220973
1102	56-57	0.03908964527246184
1102	58-59	0.08960359106715998
1102	60-61	-0.08367765065976585
1102	62-63	0.019097142979354942
1102	64-65	-0.03782258820518081
1102	66-67	-0.05231996586078225
1102	68-69	-0.07618834658521223
1102	70-71	-0.10476743231165386
1102	72-73	-0.2036455146384739
1102	74-75	-0.21885697854411035
1102	76-77	-0.12365918843097745
1102	78-79	-0.12742991780731927
1102	80-81	0.0023868186498816613
1102	82-83	0.11324574309567481
1102	84-85	0.07070724060482547
1102	86-87	-0.06494302757810999
1102	88-89	-0.09600275590884877
1102	90-91	-0.10522442340608862
1102	92-93	-0.13318373710152187
1102	94-95	-0.12061657279862104
1102	96-97	-0.0989880262875964
1102	98-99	-0.2323823016688138
1102	100	0.0387013748395546
1103	1	0.10338749882463105
1103	2	0.1733857197663582
1103	3	0.11510022772622364
1103	4	0.08443212563362579
1103	5	0.04398528915394451
1103	6	0.030413594314580905
1103	7	0.11472676327348097
1103	8	0.0684441580086883
1103	9	0.03633158414475446
1103	10-11	0.15287735718328577
1103	12-13	0.10375799851600931
1103	14-15	0.11583958820118312
1103	16-17	0.06555018302934457
1103	18-19	0.13636815255955526
1103	20-21	0.1111293654007568
1103	22-23	0.13389860026870082
1103	24-25	0.08972439482113259
1103	26-27	0.10736583399844335
1103	28-29	0.17244946254661642
1103	30-31	0.11423338875928835
1103	32-33	-0.009512062452166958
1103	34-35	0.06035333203011106
1103	36-37	0.06959888128405822
1103	38-39	0.0821355752502484
1103	40-41	0.15695456423275544
1103	42-43	0.15558389059927435
1103	44-45	0.1018655292771129
1103	46-47	0.14519173584259448
1103	48-49	0.04493442092484656
1103	50-51	0.12351380815301383
1103	52-53	0.11313105407522528
1103	54-55	0.14610163722231562
1103	56-57	0.20716986714454322
1103	58-59	0.09390741624685006
1103	60-61	0.0751791780171942
1103	62-63	0.07931680908921379
1103	64-65	-0.03457821647298687
1103	66-67	-0.06357293668786212
1103	68-69	-0.06913821273809617
1103	70-71	-0.1516325832885954
1103	72-73	-0.02676778626905474
1103	74-75	-0.04245584066543984
1103	76-77	0.013004309073885167
1103	78-79	0.04126227242458924
1103	80-81	0.03586071626177301
1103	82-83	-0.05304214792430173
1103	84-85	0.05394051537785316
1103	86-87	0.08780481184896871
1103	88-89	0.0867977122939152
1103	90-91	0.03228778597499016
1103	92-93	-0.04066894220444439
1103	94-95	0.16406467480825526
1103	96-97	0.021768445785266266
1103	98-99	0.03225551430199758
1103	100	0.2128348075953781
1104	1	-0.031250262559929354
1104	2	0.0013598592973380619
1104	3	-8.091896224300399E-4
1104	4	-0.061484408831134374
1104	5	-0.027350162637681308
1104	6	-0.036519332111218716
1104	7	-0.059021003549787565
1104	8	-0.10501287220112943
1104	9	-0.06882525869608713
1104	10-11	0.023302350687508522
1104	12-13	-0.014399200987575966
1104	14-15	-0.06798160111572571
1104	16-17	-0.06984980373701433
1104	18-19	-0.08752663677542216
1104	20-21	-0.04059254095348308
1104	22-23	-0.048346131693612904
1104	24-25	-0.057906380169868044
1104	26-27	-0.11802782644248566
1104	28-29	-0.11891155276405385
1104	30-31	-0.10500400660215803
1104	32-33	-0.07663215612051033
1104	34-35	-0.07296828738008543
1104	36-37	-0.034368789901954244
1104	38-39	-0.13547852051288345
1104	40-41	-0.2118084777614584
1104	42-43	-0.12956929685200436
1104	44-45	-0.13017557408569047
1104	46-47	-0.12227319904628331
1104	48-49	-0.16670545300053163
1104	50-51	-0.1943515653826111
1104	52-53	-0.18144513053726996
1104	54-55	-0.1956850736210356
1104	56-57	-0.0995710585324261
1104	58-59	-0.12519251871464832
1104	60-61	-0.12729817551304023
1104	62-63	-0.1167918148865894
1104	64-65	-0.14926102064900704
1104	66-67	-0.10082180012799569
1104	68-69	-0.16193289927028331
1104	70-71	-0.20067875255330136
1104	72-73	-0.12421308856896474
1104	74-75	-0.061713570644307936
1104	76-77	-0.12906264830249725
1104	78-79	0.004015870118706744
1104	80-81	0.06709537451916248
1104	82-83	0.02065006181921092
1104	84-85	-0.03914373772034452
1104	86-87	0.022339205594946066
1104	88-89	0.042794568497505736
1104	90-91	0.05586799393680408
1104	92-93	0.04302547852524796
1104	94-95	-0.032297930153067256
1104	96-97	-0.088891089778528
1104	98-99	-0.18242063270760056
1104	100	-0.10135932021898952
1105	1	0.024956126275611723
1105	2	6.388077130594638E-4
1105	3	0.04233517553209509
1105	4	0.02147594231925609
1105	5	0.015647090964655774
1105	6	0.06359685921011504
1105	7	0.041064598659943385
1105	8	0.028047713476290426
1105	9	0.0028679716181656545
1105	10-11	0.04208067517996028
1105	12-13	0.09614243757337704
1105	14-15	0.180255228300787
1105	16-17	0.0653209576133591
1105	18-19	0.02617009520373159
1105	20-21	0.06308767605891319
1105	22-23	0.03461760948719217
1105	24-25	0.013689169965843462
1105	26-27	-0.043301228453103136
1105	28-29	-0.03423600390237169
1105	30-31	-0.04212308149912758
1105	32-33	-0.026499479790835778
1105	34-35	-0.03793819652411656
1105	36-37	-0.012079576474150144
1105	38-39	-0.01631591636405716
1105	40-41	-0.060768353361531524
1105	42-43	-0.0917750698057418
1105	44-45	-0.03311525866464393
1105	46-47	0.002267215319506022
1105	48-49	0.024058417032961188
1105	50-51	-0.03109791037796583
1105	52-53	-0.12960876683210643
1105	54-55	-0.053119985825560434
1105	56-57	0.0035814174669397403
1105	58-59	-0.06427296271718319
1105	60-61	-0.09097131918757384
1105	62-63	-0.06190418729924829
1105	64-65	-0.09430050740786555
1105	66-67	-0.1263213329345021
1105	68-69	-0.19168640779408008
1105	70-71	-0.10672748920722341
1105	72-73	-0.14132442473604812
1105	74-75	-0.15779085608456
1105	76-77	-0.16109981533449513
1105	78-79	-0.07592798467067752
1105	80-81	-0.04665713528591198
1105	82-83	0.04405707864605546
1105	84-85	0.01896962205837127
1105	86-87	0.06131690802470757
1105	88-89	0.05910266455236268
1105	90-91	0.020737463575215997
1105	92-93	0.019533237910660972
1105	94-95	0.07166539381747583
1105	96-97	0.0529958736782028
1105	98-99	-0.16162796002970126
1105	100	-0.09553769195111883
1106	1	0.005037095067876862
1106	2	-0.013785623640352185
1106	3	-0.051255289931226855
1106	4	-0.016250391833594335
1106	5	-0.021374595943441932
1106	6	-0.03537112109090401
1106	7	-0.016632439814237898
1106	8	-0.0017440118187508347
1106	9	0.05921921934067598
1106	10-11	-0.06703927826691825
1106	12-13	0.03662020092288287
1106	14-15	-0.02505384446754988
1106	16-17	-0.04664532953451328
1106	18-19	-0.02898938886198721
1106	20-21	0.0014909203671891191
1106	22-23	0.008233943564476931
1106	24-25	0.009591461527229228
1106	26-27	-0.002595218015144951
1106	28-29	0.044663154222782
1106	30-31	-8.616804982892745E-4
1106	32-33	0.03307061364285602
1106	34-35	0.013731291555608038
1106	36-37	-0.07870564501804012
1106	38-39	0.017052282963796017
1106	40-41	0.06568167091415233
1106	42-43	0.0026487295633543795
1106	44-45	0.018997877823892395
1106	46-47	0.04868123480766684
1106	48-49	-0.05825551720602107
1106	50-51	-0.05698262926439668
1106	52-53	-0.08675324646057447
1106	54-55	-0.10685574967286016
1106	56-57	-0.05755558490169932
1106	58-59	-0.10798818783754882
1106	60-61	-0.15091983994356895
1106	62-63	-0.0761327645766201
1106	64-65	-0.09077116505547878
1106	66-67	-0.13220435889676452
1106	68-69	-0.128514576220347
1106	70-71	-0.2224586626196725
1106	72-73	-0.14552902858912375
1106	74-75	-0.05133805962648097
1106	76-77	-0.11449341463094953
1106	78-79	0.07456289845946884
1106	80-81	-0.016954292325848996
1106	82-83	-0.09074857205698095
1106	84-85	-0.04080273688642677
1106	86-87	-0.07568778858046699
1106	88-89	-0.03570196642718315
1106	90-91	0.08694636904326103
1106	92-93	0.07353736611158013
1106	94-95	0.052669761912920166
1106	96-97	0.08678802915001071
1106	98-99	-0.08972718858904116
1106	100	-0.10084964223257487
1107	1	-0.023095639339231866
1107	2	-0.03279620461799482
1107	3	-0.019349326209031403
1107	4	-0.0029212648612784164
1107	5	0.017464733610438543
1107	6	-0.049602503680219456
1107	7	-0.028101339890035604
1107	8	-0.017569024064435723
1107	9	-0.028426589577243533
1107	10-11	-0.06975729115531948
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1315	4	-0.1763946027715093
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2316	46-47	-0.29224439936865565
2316	48-49	-0.4246654945478028
2316	50-51	-0.3031213645920232
2316	52-53	-0.35924274414822577
2316	54-55	-0.4628827612111479
2316	56-57	-0.539299896716841
2316	58-59	-0.3883112255177821
2316	60-61	-0.2635940684562854
2316	62-63	-0.3678669477259007
2316	64-65	-0.38371508150672184
2316	66-67	-0.44461810347650754
2316	68-69	-0.3104540141282257
2316	70-71	-0.24212987427207366
2316	72-73	-0.20464952460658736
2316	74-75	-0.2251846266800328
2316	76-77	-0.17497732557830403
2316	78-79	-0.20675537923989395
2316	80-81	-0.3209551337006218
2316	82-83	-0.284560787869907
2316	84-85	-0.2607921300999507
2316	86-87	-0.24707607386145014
2316	88-89	-0.3858216177648828
2316	90-91	-0.3786269633127759
2316	92-93	-0.35393408601124676
2316	94-95	-0.37054219365843366
2316	96-97	-0.4709145197470015
2316	98-99	-0.3293223915483061
2316	100	-0.39214510748249864
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	34.0
14	172.0
15	638.0
16	1604.0
17	3507.0
18	6691.0
19	9989.0
20	14680.0
21	20820.0
22	25620.0
23	26607.0
24	25320.0
25	26169.0
26	30490.0
27	36975.0
28	45510.0
29	55766.0
30	68649.0
31	85673.0
32	109301.0
33	145382.0
34	207019.0
35	304820.0
36	511564.0
37	847717.0
38	795237.0
39	134899.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07730918136907	20.820054384849062	13.793772516784855	24.308863916997016
2	15.25934413179399	21.65884310762669	39.16228736628691	23.919525394292403
3	17.895703009164464	26.452970331982435	30.48426225341373	25.167064405439373
4	12.43338426301178	17.772189006532365	37.97188794717623	31.822538783279626
5	12.880018618229144	38.00784948155914	35.322102142678	13.790029757533716
6	30.745646921602933	37.865109993688016	17.68569157145742	13.70355151325163
7	26.5823199498653	32.74711998014042	22.74751818837283	17.923041881621447
8	25.574706962572662	35.78982419731385	20.206548244769216	18.428920595344277
9	26.047906643753116	15.28313156887692	20.71892443752833	37.95003734984164
10-11	24.753595544378783	26.248480072230723	28.59702353273929	20.4009008506512
12-13	25.259755086548314	24.035809141983915	28.608975697731935	22.095460073735833
14-15	22.55323968069253	25.535093899050526	26.3141440170942	25.597522403162742
16-17	21.915975419108328	28.169081108498297	27.214050290598625	22.700893181794754
18-19	21.619427915894605	27.42745159839055	29.384316107774332	21.568804377940506
20-21	22.56329753381684	26.763203969412658	29.097611848365794	21.575886648404712
22-23	22.465912662874903	26.62548706786627	28.816625249835422	22.09197501942341
24-25	22.111006051650843	26.666915194422135	29.21699353714869	22.005085216778333
26-27	22.108127350888516	26.88722414370362	28.836817394185037	22.167831111222824
28-29	21.87777385219885	27.084658922261955	28.992730336167938	22.044836889371254
30-31	22.429539263438876	26.76657197578131	28.965229659405118	21.838659101374695
32-33	22.374115531234803	26.989239234009236	28.60504865248956	22.031596582266396
34-35	22.405647555346377	27.045594438299513	28.85782392664832	21.69093407970579
36-37	22.385398988516812	26.86989813714135	28.66730438628524	22.077398488056595
38-39	22.278531669156887	26.9482854472393	28.902985313839146	21.870197569764667
40-41	22.328978767732917	26.606964904350512	29.03444776717132	22.029608560745253
42-43	22.14609434772773	26.721025160467278	28.69720084964978	22.435679642155215
44-45	22.160074266877245	27.13367586021469	28.601248777093176	22.10500109581489
46-47	22.214032009328935	26.971754921152964	28.18939952042067	22.624813549097432
48-49	22.440494809778983	26.927026219777844	28.279851802790667	22.352627167652507
50-51	22.406534292424528	27.069712045282163	27.987836109452985	22.535917552840324
52-53	22.090511611066347	27.28887962522845	27.963375779538442	22.657232984166768
54-55	22.089085346273084	27.207836717817322	27.97611331264261	22.726964623266984
56-57	22.4364287018482	27.100260861448582	27.711116951023484	22.752193485679733
58-59	22.062457335281355	27.184186467662087	28.08501396197181	22.668342235084754
60-61	22.25168527584013	27.34570555634403	27.808509451454803	22.594099716361033
62-63	22.223629637568894	27.114812189836137	27.768172991774808	22.893385180820157
64-65	21.86527755497665	27.056583076704086	27.902894732680068	23.175244635639196
66-67	22.067736769365702	26.98442039336011	27.745276036817618	23.20256680045657
68-69	21.899297463902727	26.976886250324604	27.85342186098707	23.270394424785596
70-71	21.917426173815226	26.738153679567482	27.860758481951592	23.483661664665696
72-73	21.976730392835254	26.856357695012527	27.777115630657473	23.389796281494746
74-75	22.025244738278815	26.96839168518273	27.6086610844036	23.39770249213485
76-77	21.91191401809506	26.849617806422092	27.589913987028154	23.64855418845469
78-79	21.76479351668734	26.843134250691765	27.67077616317862	23.72129606944228
80-81	21.601237234697983	27.06990328031847	27.574662163164	23.75419732181955
82-83	21.953084577339247	26.708822971199016	27.88961655169921	23.448475899762535
84-85	21.99119493640356	26.816822791775934	27.477859192129696	23.71412307969081
86-87	21.48942296436034	27.048228763284044	27.69499659748238	23.76735167487324
88-89	21.685225680603086	26.539757070796504	27.662250516610765	24.11276673198965
90-91	21.68306565275797	26.505001597794408	27.65658561713909	24.15534713230853
92-93	21.71260671420095	26.529456070703265	27.587313672888936	24.170623542206844
94-95	21.289236806966095	26.44935845151728	27.792766509790983	24.468638231725638
96-97	21.348146106351695	26.36332903865717	27.731558074998492	24.556966779992646
98-99	21.192146028914667	26.493299740484794	27.57202895476855	24.742525275831987
100	21.00232597283616	26.43517833399795	27.517619893789234	25.044875799376655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	817.0
1	558.0
2	433.0
3	581.0
4	496.0
5	472.0
6	725.5
7	1294.5
8	2014.0
9	2398.0
10	2198.5
11	1924.5
12	2057.0
13	2399.0
14	2755.5
15	3101.0
16	3453.0
17	3855.5
18	4319.0
19	4764.0
20	5359.0
21	6216.0
22	7203.0
23	8503.0
24	10310.5
25	12777.0
26	15894.0
27	19411.0
28	24004.0
29	29835.5
30	37000.5
31	44509.5
32	53088.0
33	62129.5
34	70235.0
35	78394.5
36	87136.5
37	98038.5
38	104916.0
39	106990.5
40	107904.5
41	108026.5
42	110679.0
43	115838.0
44	121963.0
45	131268.0
46	139792.5
47	144878.0
48	149348.5
49	150634.5
50	147474.0
51	145042.5
52	141599.0
53	134704.5
54	126985.0
55	114146.0
56	100745.5
57	86741.5
58	72342.5
59	62806.5
60	51926.5
61	40118.5
62	31011.5
63	23693.5
64	17041.0
65	11542.0
66	8090.0
67	5883.5
68	4501.5
69	3374.5
70	2450.5
71	1818.5
72	1365.5
73	1018.0
74	749.0
75	485.5
76	328.0
77	196.0
78	101.5
79	51.5
80	26.0
81	17.0
82	8.5
83	4.0
84	2.0
85	0.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005450614747443083
2	0.026434069448739512
3	9.884534516088493E-4
4	0.0015532839953853344
5	0.007596970813793727
6	0.0
7	6.777966525317823E-4
8	0.005958962236841919
9	0.0011296610875529706
10-11	2.682945082938305E-4
12-13	4.2362290783236396E-5
14-15	9.743326880144372E-4
16-17	1.2708687234970917E-4
18-19	0.001186144141930619
20-21	2.4005298110500622E-4
22-23	0.0015250424681965103
24-25	9.319703972312007E-4
26-27	0.0019627861396232862
28-29	1.6944916313294558E-4
30-31	0.0018498200308679892
32-33	0.0016662501041406316
34-35	7.201589433150187E-4
36-37	0.004066779915190694
38-39	9.743326880144372E-4
40-41	0.0033466209718756752
42-43	0.0037278815889248028
44-45	0.003982055333624221
46-47	0.002075752248378583
48-49	0.009390307790284068
50-51	0.00682032881610106
52-53	0.004744576567722476
54-55	0.004744576567722476
56-57	0.003290137917498027
58-59	0.005535339329009556
60-61	0.008260646702731095
62-63	0.0032618963903092027
64-65	0.0030783264635818445
66-67	0.0037984854068968633
68-69	0.0019910276668121106
70-71	0.0028806357732600748
72-73	0.0060719283455972165
74-75	0.007512246232227253
76-77	0.003953813806435397
78-79	0.002894756536854487
80-81	0.0024146505746444748
82-83	0.004885784203666597
84-85	0.0023016844658891775
86-87	0.0035160701350086206
88-89	5.083474893988367E-4
90-91	0.0018780615580568133
92-93	5.648305437764853E-5
94-95	0.0021745975935394683
96-97	0.0018921823216512255
98-99	0.0014685594138188617
100	2.8241527188824265E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3540885.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.7653161762061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33853989730608	58.37286033041125
2	12.54405035256045	18.00455479363501
3	3.2146559177723675	6.921023950099378
4	1.1764389715533912	3.3771005902215943
5	0.545368203904336	1.9569260792822156
6	0.3135895528499695	1.3502912045899893
7	0.20008668386381698	1.0051498891094794
8	0.13508935446140477	0.7755784187969832
9	0.09023657624710872	0.5828270782528877
>10	0.41943940006008246	4.980863669273317
>50	0.016149551146225346	0.7929348620248234
>100	0.005660567533988232	0.738130397942053
>500	4.34356715054296E-4	0.20367619429039607
>1k	1.3030701287435672E-4	0.14999844462163892
>5k	8.687134191623781E-5	0.47293773935983074
>10k+	4.3435670958118905E-5	0.3151463580891042
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10110	0.2855218398790133	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9139	0.25809931697866495	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6033	0.17038113353017678	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.8241527188824262E-5	0.0	0.0	0.0	0.0
7	2.8241527188824262E-5	0.0	0.0	0.0	0.0
8	2.8241527188824262E-5	0.0	0.0	0.0	0.0
9	2.8241527188824262E-5	0.0	0.0	1.1296610875529705E-4	0.0
10-11	2.8241527188824262E-5	0.0	0.0	1.1296610875529705E-4	0.0
12-13	2.8241527188824262E-5	0.0	0.0	1.1296610875529705E-4	2.8241527188824262E-5
14-15	2.8241527188824262E-5	0.0	0.0	1.9769069032176984E-4	5.6483054377648524E-5
16-17	5.6483054377648524E-5	0.0	0.0	1.9769069032176984E-4	1.1296610875529705E-4
18-19	5.6483054377648524E-5	0.0	0.0	1.9769069032176984E-4	1.9769069032176984E-4
20-21	5.6483054377648524E-5	0.0	0.0	3.812606170491275E-4	2.259322175105941E-4
22-23	5.6483054377648524E-5	0.0	0.0	4.942267258044245E-4	2.4005298110500622E-4
24-25	5.6483054377648524E-5	0.0	0.0	0.0010166949787976734	2.682945082938305E-4
26-27	5.6483054377648524E-5	0.0	0.0	0.0019204238488400498	2.824152718882426E-4
28-29	5.6483054377648524E-5	0.0	0.0	0.005394131693065435	2.824152718882426E-4
30-31	5.6483054377648524E-5	0.0	0.0	0.017665075256609576	2.824152718882426E-4
32-33	5.6483054377648524E-5	0.0	0.0	0.048278890729295074	2.824152718882426E-4
34-35	5.6483054377648524E-5	0.0	0.0	0.0997631947945217	3.106567990770669E-4
36-37	5.6483054377648524E-5	0.0	0.0	0.1819460389140003	3.106567990770669E-4
38-39	5.6483054377648524E-5	0.0	0.0	0.29765157580661333	3.106567990770669E-4
40-41	5.6483054377648524E-5	0.0	0.0	0.4193725579904459	3.3889832626589117E-4
42-43	5.6483054377648524E-5	0.0	0.0	0.5463747057585886	3.530190898603033E-4
44-45	7.060381797206066E-5	0.0	0.0	0.7060240589570121	3.671398534547154E-4
46-47	8.472458156647279E-5	0.0	0.0	0.8976004586424016	3.953813806435397E-4
48-49	8.472458156647279E-5	0.0	0.0	1.1034388295581472	4.2362290783236393E-4
50-51	8.472458156647279E-5	0.0	0.0	1.3116494887577541	4.518644350211882E-4
52-53	8.472458156647279E-5	0.0	0.0	1.517191323638017	5.083474893988367E-4
54-55	8.472458156647279E-5	0.0	0.0	1.7536293892628536	5.648305437764852E-4
56-57	8.472458156647279E-5	0.0	0.0	2.034378411046956	5.648305437764852E-4
58-59	8.472458156647279E-5	0.0	0.0	2.3468426678641077	5.648305437764852E-4
60-61	8.472458156647279E-5	0.0	0.0	2.663684361395527	5.648305437764852E-4
62-63	1.1296610875529705E-4	0.0	0.0	2.9882501126130894	5.648305437764852E-4
64-65	1.412076359441213E-4	0.0	0.0	3.3251009281577915	5.648305437764852E-4
66-67	1.412076359441213E-4	0.0	0.0	3.707349998658527	5.648305437764852E-4
68-69	1.412076359441213E-4	0.0	0.0	4.133599368519452	5.648305437764852E-4
70-71	1.412076359441213E-4	0.0	0.0	4.58786433335169	5.648305437764852E-4
72-73	1.412076359441213E-4	0.0	0.0	5.036396268164597	5.930720709653095E-4
74-75	1.412076359441213E-4	0.0	0.0	5.488839654493157	5.930720709653095E-4
76-77	1.412076359441213E-4	0.0	0.0	5.985311581709093	5.930720709653095E-4
78-79	1.412076359441213E-4	0.0	0.0	6.535823671200843	5.930720709653095E-4
80-81	1.412076359441213E-4	0.0	0.0	7.115085635370818	6.071928345597217E-4
82-83	1.412076359441213E-4	0.0	0.0	7.704330979402043	6.777966525317823E-4
84-85	1.412076359441213E-4	0.0	0.0	8.279469680602448	6.777966525317823E-4
86-87	1.412076359441213E-4	0.0	0.0	8.872668838440108	6.919174161261945E-4
88	1.412076359441213E-4	0.0	0.0	9.342184227954311	7.060381797206065E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5255	0.0	55.821247	1
GTATCAA	9540	0.0	41.047268	1
CAACGCA	12000	0.0	31.881954	5
ATCAACG	12060	0.0	31.724234	3
TATCAAC	12660	0.0	30.560894	2
GTGGTAT	1945	0.0	30.211903	1
TCAACGC	12830	0.0	30.003456	4
AACGCAG	12995	0.0	29.800817	6
ACGCAGA	14515	0.0	26.259172	7
CGCAGAG	15060	0.0	25.215628	8
GTACATG	10955	0.0	25.10332	1
TACATGG	11190	0.0	24.240726	2
TGGTATC	2285	0.0	24.071302	2
ACATGGG	10960	0.0	23.972609	3
GTACTAG	1515	0.0	23.582434	1
CTGTGCG	1930	0.0	22.888811	9
GCAGAGT	16480	0.0	22.413933	9
CTAGTAC	2110	0.0	22.275711	3
CATGGGG	8485	0.0	21.603632	4
TAACGCC	665	0.0	21.203798	4
>>END_MODULE