FastQCFastQC Report
Thu 26 May 2016
ERR580179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7440184
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176010.23656673007011655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141280.18988777696895667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86130.11576326606976388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111200.047.068841
GTATCAA193200.039.2374731
CAACGCA216750.033.2797555
GTACTAG54300.032.4768941
ATCAACG223450.032.285363
TATCAAC243650.030.9791322
AACGCAG247500.029.7763526
GCATCGT55550.028.6810998
TCAACGC256500.028.4177934
CTGTGCG75850.028.380839
TAGTACT77200.028.2039364
GTACATG274300.027.5678771
ACGCAGA263500.027.2901297
TAGGCAT68250.027.2149965
TACATGG276050.026.8831312
GGGCACC72250.026.54134694
GTGGTAT38650.026.5246751
CGCAGAG271950.026.4240048
ACATGGG271550.026.3068333
CTAGTAC87000.025.4602263
Produced by FastQC (version 0.11.5)