FastQCFastQC Report
Thu 26 May 2016
ERR580179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7440184
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155290.2087179564376365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127220.17099039486120235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81780.10991663647028085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107450.044.4517861
GTATCAA187450.039.6003151
CAACGCA214650.032.8436475
GTACTAG51700.032.826051
ATCAACG221100.031.9072073
TATCAAC243850.030.3388582
AACGCAG245750.029.5659856
TCAACGC253550.028.323944
GTGGTAT34800.028.2337761
CTGTGCG77400.027.5065789
TAGTACT76800.027.110434
ACGCAGA264400.026.662657
GTACATG263950.026.3241711
TAGGCAT68050.026.1759265
CGCAGAG272850.025.7854238
CTAGTAC86900.025.365883
GGGCACC71650.025.25923394
TACATGG273600.025.1502482
ACATGGG267200.024.748823
GTACTGT93950.023.911546
Produced by FastQC (version 0.11.5)