FastQCFastQC Report
Thu 26 May 2016
ERR580178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9833865
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201640.20504654070398567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159300.16199124149050248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138700.043.678851
GTATCAA238850.039.8581431
GTACTAG77850.035.3450851
CAACGCA264850.033.9341665
ATCAACG277050.032.5468563
TATCAAC300700.031.2855512
GCATCGT81450.030.9883798
AACGCAG311250.029.6588556
GTACATG377500.028.6204091
TAGGCAT100850.028.3999715
TCAACGC322500.028.3086224
GGGCACC97950.028.11657794
TACATGG386750.027.547692
CTGTGCG114100.027.3943219
TAGTACT114300.027.3651854
TCTAACG38100.027.160952
ACGCAGA329550.027.1132097
CTAGGCA109350.026.8833984
CTAACGC38150.026.8786223
ACATGGG384150.026.864633
Produced by FastQC (version 0.11.5)