FastQCFastQC Report
Thu 26 May 2016
ERR580178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9833865
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169360.1722211968539328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140070.14243636657611222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142950.042.028211
GTATCAA241450.037.9081761
GTACTAG73900.033.5879631
CAACGCA267600.032.4752275
ATCAACG281600.030.9276143
GGGCACC94200.030.14103394
TATCAAC309450.029.4360942
TAGTACT109200.028.8801294
AACGCAG314250.028.4905786
CTGTGCG109200.028.4485669
GTACATG367100.027.3533841
TCAACGC323050.027.3373414
CTAGTAC119050.027.2410163
TAGGCAT96450.027.0453245
GTGGTAT48250.026.9883691
TACATGG377200.026.3296282
ACGCAGA330700.026.192117
ACATGGG373650.025.3965643
CTAGGCA106800.025.2163724
CGCAGAG343450.025.192538
Produced by FastQC (version 0.11.5)