FastQCFastQC Report
Thu 26 May 2016
ERR580177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6450417
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186570.28923711443771777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150430.23320972892140152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90050.13960337758008515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110200.051.034021
GTATCAA202350.041.2947041
CAACGCA237200.033.809575
ATCAACG240950.033.3785973
TATCAAC258550.031.8924082
AACGCAG265000.030.849126
GTACTAG49500.029.9237171
GTGGTAT36100.029.3079551
TCAACGC276200.029.2902054
ACGCAGA283600.028.0308917
CGCAGAG286600.027.671698
GTACATG313100.026.7029251
TAGTACT64900.026.5819344
GCATCGT49850.026.5780648
CTGTGCG65600.026.4307279
TAGGCAT60350.026.0941315
TACATGG320450.025.7318842
ACATGGG315500.025.4617163
GGGCACC58400.025.43387294
GCAGAGT311450.024.9385419
Produced by FastQC (version 0.11.5)