FastQCFastQC Report
Thu 26 May 2016
ERR580177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6450417
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161650.25060395320178525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134970.2092422861963808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84260.13062721371347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109250.052.8065151
GTATCAA199950.041.9975971
CAACGCA238500.033.601265
ATCAACG241900.033.148933
TATCAAC260450.032.0985372
AACGCAG264150.030.9585936
TCAACGC277950.029.1536924
GTACTAG46150.028.730411
GTGGTAT33950.028.3908581
ACGCAGA285850.027.9342987
CGCAGAG290400.027.4642528
GTACATG311950.026.4820231
TACATGG315850.025.9474322
ACATGGG308500.025.4441323
GCAGAGT319250.024.7607739
TAGGCAT58700.024.5821515
GGGCACC57700.024.27278794
TATTCTC33550.023.3961055
TAGTACT66950.023.3784984
CTGTGCG67450.023.202329
Produced by FastQC (version 0.11.5)