##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580177_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6450417
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52282464839095	34.0	31.0	34.0	31.0	34.0
2	32.64430067079384	34.0	31.0	34.0	31.0	34.0
3	32.69627731044365	34.0	31.0	34.0	31.0	34.0
4	36.21421715836356	37.0	35.0	37.0	35.0	37.0
5	36.158788183771684	37.0	35.0	37.0	35.0	37.0
6	36.1966646497428	37.0	37.0	37.0	35.0	37.0
7	36.14434834213044	37.0	36.0	37.0	35.0	37.0
8	36.140863296124884	37.0	36.0	37.0	35.0	37.0
9	37.8541948528289	39.0	38.0	39.0	35.0	39.0
10-11	37.80696643333292	39.0	38.0	39.0	35.0	39.0
12-13	37.763379251294914	39.0	38.0	39.0	35.0	39.0
14-15	39.13268080187684	40.0	38.5	41.0	36.0	41.0
16-17	39.125893798804015	40.0	38.0	41.0	36.0	41.0
18-19	39.121177824627466	40.0	38.5	41.0	35.5	41.0
20-21	39.09449249560144	40.0	39.0	41.0	35.0	41.0
22-23	39.01527707433489	40.0	38.5	41.0	35.0	41.0
24-25	38.93854722880707	40.0	38.0	41.0	35.0	41.0
26-27	38.80936589060832	40.0	38.0	41.0	35.0	41.0
28-29	38.71335078026739	40.0	38.0	41.0	35.0	41.0
30-31	38.56596387489367	40.0	38.0	41.0	34.0	41.0
32-33	38.46266194883214	40.0	38.0	41.0	34.0	41.0
34-35	38.37126669485089	40.0	38.0	41.0	34.0	41.0
36-37	38.25317812786368	40.0	38.0	41.0	34.0	41.0
38-39	38.137177255361934	40.0	38.0	41.0	34.0	41.0
40-41	38.02609381688036	40.0	37.0	41.0	33.0	41.0
42-43	38.028927122075984	40.0	37.0	41.0	33.0	41.0
44-45	37.978823539625424	40.0	37.0	41.0	33.0	41.0
46-47	37.787421185328014	40.0	37.0	41.0	33.0	41.0
48-49	37.57647784631598	40.0	37.0	41.0	33.0	41.0
50-51	37.37705438268564	40.0	36.0	41.0	32.5	41.0
52-53	37.20203941853681	39.5	36.0	41.0	32.0	41.0
54-55	36.978877179568386	39.0	35.0	41.0	32.0	41.0
56-57	36.7287147482093	39.0	35.0	41.0	31.0	41.0
58-59	36.46933066497871	38.5	35.0	40.5	31.0	41.0
60-61	36.207458603063955	38.0	35.0	40.0	31.0	41.0
62-63	35.896061138372914	37.0	35.0	40.0	30.0	41.0
64-65	35.59786971912048	37.0	35.0	40.0	30.0	41.0
66-67	35.28434115189762	36.0	34.0	39.5	29.5	41.0
68-69	34.97554979778827	36.0	34.0	39.0	29.0	41.0
70-71	34.674222302217046	35.0	34.0	39.0	29.0	40.5
72-73	34.37550432785973	35.0	34.0	38.0	29.0	40.0
74-75	34.03640051488144	35.0	34.0	37.0	29.0	39.5
76-77	32.522163373313695	34.0	31.5	35.5	26.5	38.5
78-79	33.422136894405426	35.0	33.0	36.0	28.5	39.0
80-81	33.416063798666045	35.0	33.0	36.0	29.0	38.0
82-83	33.19775574199312	35.0	33.0	35.5	28.5	37.0
84-85	32.99859605975861	35.0	33.0	35.0	28.0	37.0
86-87	32.80944262983308	35.0	33.0	35.0	27.0	36.5
88-89	32.64210445619252	35.0	33.0	35.0	27.0	36.0
90-91	32.480127021245295	35.0	33.0	35.0	27.0	36.0
92-93	32.31998845966083	35.0	33.0	35.0	26.0	35.5
94-95	32.195620298656664	35.0	33.0	35.0	26.0	35.0
96-97	32.07937052751784	35.0	32.5	35.0	25.0	35.0
98-99	31.980018423615093	35.0	32.0	35.0	25.0	35.0
100	30.080011106258713	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07829055024882337
1101	2	-0.07649792826723001
1101	3	-0.05641352309770298
1101	4	-0.016597997187730584
1101	5	-0.06357035526330179
1101	6	-0.023360934047751414
1101	7	-0.05874915961301497
1101	8	-0.04662610795667632
1101	9	-0.10069710324542314
1101	10-11	-0.0537870108553804
1101	12-13	-0.03318411724806225
1101	14-15	-0.06920442722088893
1101	16-17	-0.06877697824172913
1101	18-19	-0.054311045620906384
1101	20-21	-0.07544939893244162
1101	22-23	-0.07632869703872558
1101	24-25	-0.10841875202159912
1101	26-27	-0.08421278491249495
1101	28-29	-0.06743304335831368
1101	30-31	-0.1543367678561154
1101	32-33	-0.17758233590605954
1101	34-35	-0.15786298059427395
1101	36-37	-0.1815247248572689
1101	38-39	-0.1683710446723765
1101	40-41	-0.15366426821477575
1101	42-43	-0.13184349832456377
1101	44-45	-0.07195480943530441
1101	46-47	-0.11402570734262696
1101	48-49	-0.11702336903066879
1101	50-51	-0.0865338000657161
1101	52-53	-0.06746218220494171
1101	54-55	-0.0732524187284227
1101	56-57	-0.14054433463937244
1101	58-59	-0.18248623708667822
1101	60-61	-0.15839191580248269
1101	62-63	-0.09930999956063147
1101	64-65	-0.1307553976813054
1101	66-67	-0.19587914357803982
1101	68-69	-0.2003357954755529
1101	70-71	-0.17741349142367113
1101	72-73	-0.1678879530165176
1101	74-75	-0.18553434061249874
1101	76-77	-0.22599711692751612
1101	78-79	-0.16299442837171085
1101	80-81	-0.12310433011631972
1101	82-83	-0.13505931562582418
1101	84-85	-0.1404262531100713
1101	86-87	-0.1771823389393603
1101	88-89	-0.16297650736408542
1101	90-91	-0.17987621628574146
1101	92-93	-0.20028220632740812
1101	94-95	-0.22398258553110395
1101	96-97	-0.2690603342694544
1101	98-99	-0.26858804161039274
1101	100	-0.5340481488120119
1102	1	-0.03242345362164656
1102	2	-0.0638411094916762
1102	3	-0.024151926190285167
1102	4	-0.02834701924622607
1102	5	0.005104990022836375
1102	6	-0.024319253426931198
1102	7	0.006988649636888056
1102	8	-0.034408888130158743
1102	9	-0.0023626248084696044
1102	10-11	0.03663350686955624
1102	12-13	-0.008200387501723583
1102	14-15	-0.02127722301150925
1102	16-17	0.009623476001884512
1102	18-19	-0.04574165694511123
1102	20-21	-0.05335735792046847
1102	22-23	-0.056118826590797255
1102	24-25	-0.05411347890575513
1102	26-27	-0.03460397590082209
1102	28-29	-0.11246914864799606
1102	30-31	-0.12117882526781187
1102	32-33	-0.14729440276630612
1102	34-35	-0.17578514692316105
1102	36-37	-0.14058324252926724
1102	38-39	-0.16599542939496104
1102	40-41	-0.2018548750276281
1102	42-43	-0.09810384022143381
1102	44-45	-0.13026640593992056
1102	46-47	-0.0797747374571216
1102	48-49	-0.015698487022383745
1102	50-51	-0.0829554663468528
1102	52-53	-0.12481989013332395
1102	54-55	-0.09820615424055745
1102	56-57	-0.044554038673247476
1102	58-59	-0.051106686450658856
1102	60-61	-0.03560786393101978
1102	62-63	-0.08819917136875688
1102	64-65	-0.09552142251452267
1102	66-67	-0.08790886275720311
1102	68-69	-0.10413192905333091
1102	70-71	-0.14790460277261985
1102	72-73	-0.1673724549885094
1102	74-75	-0.18169364006227795
1102	76-77	-0.18607938025071036
1102	78-79	-0.14714606483411075
1102	80-81	-0.12720121163664544
1102	82-83	-0.01774391035696965
1102	84-85	-0.0815969431759811
1102	86-87	-0.11444570816795618
1102	88-89	-0.14903077088982997
1102	90-91	-0.1365080637464331
1102	92-93	-0.12283751080412486
1102	94-95	-0.11958072666541852
1102	96-97	-0.15716889354497
1102	98-99	-0.16843617152383672
1102	100	-0.024419841390244557
1103	1	0.030130789827609306
1103	2	0.012752689864605316
1103	3	0.016399357820546356
1103	4	1.584532781819803E-4
1103	5	-0.006414169904239486
1103	6	-0.01668208589303788
1103	7	0.04888441803868204
1103	8	0.006265115108391228
1103	9	0.010841875487251684
1103	10-11	0.008949300656951209
1103	12-13	0.008667331582010718
1103	14-15	8.050110543749156E-4
1103	16-17	-0.005709200539911308
1103	18-19	0.0026624669968740022
1103	20-21	0.023461445146182314
1103	22-23	-0.0293342475686984
1103	24-25	-0.026220384188221146
1103	26-27	0.013894624125320831
1103	28-29	0.01239353983344671
1103	30-31	0.005153309670177464
1103	32-33	-3.6515239471412997E-4
1103	34-35	-0.0055651051454645994
1103	36-37	-0.03888965073240058
1103	38-39	0.011686146328777625
1103	40-41	0.015905050499078754
1103	42-43	0.09762676559953576
1103	44-45	0.07150468836560009
1103	46-47	0.007354806643164125
1103	48-49	0.0015438690538829292
1103	50-51	0.01829504036837193
1103	52-53	0.013155813010200745
1103	54-55	0.0440633650943667
1103	56-57	0.0854317622247791
1103	58-59	0.019821427549743476
1103	60-61	0.006256449361870864
1103	62-63	0.04163591636220332
1103	64-65	0.022104846488680607
1103	66-67	0.035784005793665585
1103	68-69	-0.009590885674079175
1103	70-71	0.024497028019716538
1103	72-73	-0.010926317432222277
1103	74-75	0.08801453627240363
1103	76-77	0.08058861550506435
1103	78-79	0.019797689222379233
1103	80-81	0.006756239790689733
1103	82-83	-0.005544755214607733
1103	84-85	-0.002660762712260123
1103	86-87	0.07379500980598408
1103	88-89	0.087991366196853
1103	90-91	-0.027580516824045276
1103	92-93	-0.07062538042934108
1103	94-95	0.009544599792093322
1103	96-97	-0.07420632069427313
1103	98-99	-0.06696611590145451
1103	100	0.08451185893560265
1104	1	0.01415688737551335
1104	2	0.00849269744882264
1104	3	-0.013199760513892045
1104	4	0.033552360758939415
1104	5	0.028187957387004303
1104	6	-0.009719236204851711
1104	7	0.02012909568873056
1104	8	0.03099518489145936
1104	9	0.035983409807911926
1104	10-11	0.06033808363697091
1104	12-13	0.025266416941576608
1104	14-15	-0.008983887955970715
1104	16-17	-0.013352929750290343
1104	18-19	-0.007540395427852786
1104	20-21	-0.05706225760185646
1104	22-23	-0.04823111454395246
1104	24-25	0.03018967273465023
1104	26-27	-0.10508210386086603
1104	28-29	-0.10552649817008586
1104	30-31	0.008003987097644938
1104	32-33	-0.06474575039396058
1104	34-35	-0.0576594472762153
1104	36-37	-0.02723720791288997
1104	38-39	0.015759392787074944
1104	40-41	-0.06172850802513352
1104	42-43	-0.05959042852763474
1104	44-45	-0.007399252749529239
1104	46-47	0.04220978419964183
1104	48-49	0.023966651184800014
1104	50-51	0.06280467315198024
1104	52-53	-0.017114086292792763
1104	54-55	0.02589130119731209
1104	56-57	-0.022184711195414764
1104	58-59	-0.012597042086582633
1104	60-61	-0.08343949658407723
1104	62-63	-0.020753205640694716
1104	64-65	-0.001250454633698439
1104	66-67	-0.04361744365791509
1104	68-69	-0.02215644898789293
1104	70-71	0.07811419082076299
1104	72-73	0.0055974924841493134
1104	74-75	0.03351130267430591
1104	76-77	0.013322690704249851
1104	78-79	0.08345541626465547
1104	80-81	0.13479773351103574
1104	82-83	0.08108929381077701
1104	84-85	0.05094632729676363
1104	86-87	0.12798518824124727
1104	88-89	0.029140782541666965
1104	90-91	0.008920550737336441
1104	92-93	0.06735612994331319
1104	94-95	0.05888736943379058
1104	96-97	-0.026624460581906817
1104	98-99	-0.03209220355690334
1104	100	0.09250192731724027
1105	1	0.022607203238223406
1105	2	0.018744498143007604
1105	3	0.037236576743680416
1105	4	0.016337744103211094
1105	5	0.03313413422267786
1105	6	-0.0023306578637871667
1105	7	-0.029137580992774303
1105	8	0.009569790161897629
1105	9	-0.01732138782647752
1105	10-11	-0.0022598081079010512
1105	12-13	0.034304775087989015
1105	14-15	-0.010852775281136928
1105	16-17	-0.02564301347960196
1105	18-19	-0.04877159784089713
1105	20-21	-0.06293852955214163
1105	22-23	-0.05564697014053621
1105	24-25	-0.06580323913214414
1105	26-27	-0.09710291363956003
1105	28-29	-0.06674565312862057
1105	30-31	-0.07615010172343517
1105	32-33	-0.07360937597607631
1105	34-35	-0.08139367390671737
1105	36-37	-0.039364616415483056
1105	38-39	-0.11647318139520024
1105	40-41	-0.050002450734986326
1105	42-43	-0.043395101635425704
1105	44-45	0.002393825548047346
1105	46-47	-0.06887540583190344
1105	48-49	-0.08771517939577222
1105	50-51	-0.06472292036156091
1105	52-53	-0.10508323532148722
1105	54-55	-0.08725381577862379
1105	56-57	-0.11814154988010017
1105	58-59	-0.09978236534182372
1105	60-61	-0.08647275704629465
1105	62-63	0.0013377145911235289
1105	64-65	0.014241395792062406
1105	66-67	-0.04981654588041806
1105	68-69	-0.09869123934834079
1105	70-71	-0.0624452597599614
1105	72-73	-0.02382399250545575
1105	74-75	-0.03356821250212505
1105	76-77	-0.09312971443809914
1105	78-79	0.054796014308607255
1105	80-81	0.04543047689135449
1105	82-83	0.07889169579054567
1105	84-85	0.08504141422818634
1105	86-87	0.1069548195634411
1105	88-89	0.09465753828767731
1105	90-91	0.10093197160561829
1105	92-93	0.0011277936186999682
1105	94-95	0.034881407969827194
1105	96-97	0.026405785545904337
1105	98-99	0.1063074517218432
1105	100	0.20498631207676254
1106	1	-0.008105023959700475
1106	2	-0.015195162563287568
1106	3	0.019330963148654234
1106	4	0.029368356980604915
1106	5	0.04266665591056551
1106	6	-0.019920852845324077
1106	7	-0.028774968787580235
1106	8	-0.021777117864800744
1106	9	0.02077181980907028
1106	10-11	-0.014798439954972764
1106	12-13	-0.007111822304644022
1106	14-15	-0.042127433324644414
1106	16-17	-0.06612754959919442
1106	18-19	-0.0070200232534745055
1106	20-21	-0.0010724745014485393
1106	22-23	0.009898725468197256
1106	24-25	-0.01897171227543737
1106	26-27	-0.022877720513164945
1106	28-29	0.02890217087217195
1106	30-31	-0.06769178159537859
1106	32-33	-0.03158063634369768
1106	34-35	0.019851504684041288
1106	36-37	-0.035231941703592895
1106	38-39	0.016174041446809895
1106	40-41	0.06972883479217984
1106	42-43	4.4668685963245025E-4
1106	44-45	0.07179972585630168
1106	46-47	0.06595149196594718
1106	48-49	0.033236315906428615
1106	50-51	-0.01213836087871556
1106	52-53	0.06998394743224168
1106	54-55	-0.00596826671913675
1106	56-57	0.013964941437883738
1106	58-59	-0.02217824484500852
1106	60-61	-0.02003690272211145
1106	62-63	-0.007664171109105666
1106	64-65	-0.03927693998868875
1106	66-67	0.020858728248661862
1106	68-69	-0.07422981553616381
1106	70-71	-0.05021415254673656
1106	72-73	-5.461061283611457E-4
1106	74-75	-0.0952445566121014
1106	76-77	-0.08179003092319448
1106	78-79	0.03010571373991411
1106	80-81	0.08681706743071516
1106	82-83	0.04117752081091908
1106	84-85	0.02714078855792934
1106	86-87	0.03067424821154674
1106	88-89	-0.014842553618194643
1106	90-91	0.01983378597055463
1106	92-93	0.05687571036219907
1106	94-95	0.05305519793343194
1106	96-97	0.032093452614873286
1106	98-99	-0.02848251830682358
1106	100	0.12115319698541072
1107	1	0.0055993576993458305
1107	2	0.01601397470185617
1107	3	0.004164659748539634
1107	4	-0.0015104090682882543
1107	5	-0.022308544030195776
1107	6	0.01650661932607278
1107	7	-0.014028811649026807
1107	8	0.001721488364552215
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2316	9	-0.0694538135278151
2316	10-11	-0.08544351191424937
2316	12-13	-0.1650210370146965
2316	14-15	-0.2269350449887355
2316	16-17	-0.24336812283917197
2316	18-19	-0.17547411300967042
2316	20-21	-0.1305872507243251
2316	22-23	-0.1847683050104436
2316	24-25	-0.17129207769497157
2316	26-27	-0.11758054078587321
2316	28-29	-0.27823284193225817
2316	30-31	-0.23366276888759074
2316	32-33	-0.20322503920377955
2316	34-35	-0.2434372289244635
2316	36-37	-0.19327985038493978
2316	38-39	-0.21394762890304975
2316	40-41	-0.1595509212700037
2316	42-43	-0.21449078026416402
2316	44-45	-0.2787263614716693
2316	46-47	-0.2958720146406435
2316	48-49	-0.23545366682504465
2316	50-51	-0.2444107936089992
2316	52-53	-0.26463013683996195
2316	54-55	-0.30041156646471734
2316	56-57	-0.4198044606032383
2316	58-59	-0.3631414834346458
2316	60-61	-0.1814337810887352
2316	62-63	-0.267140760705594
2316	64-65	-0.2616201397321518
2316	66-67	-0.33928171926644524
2316	68-69	-0.3010273595371231
2316	70-71	-0.2646295908203413
2316	72-73	-0.264853691344193
2316	74-75	-0.25802120749907687
2316	76-77	-0.2767839601351625
2316	78-79	-0.2207017826335118
2316	80-81	-0.25014786701343894
2316	82-83	-0.2966142900075539
2316	84-85	-0.21074301735501422
2316	86-87	-0.23356057680803843
2316	88-89	-0.27391808822150665
2316	90-91	-0.3048844829000359
2316	92-93	-0.3035021009416212
2316	94-95	-0.3335168006716458
2316	96-97	-0.3325816073180299
2316	98-99	-0.1630016988355223
2316	100	-0.42746349746402146
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	67.0
14	218.0
15	720.0
16	1949.0
17	4654.0
18	8689.0
19	13735.0
20	20481.0
21	31229.0
22	40533.0
23	41205.0
24	38464.0
25	38629.0
26	43942.0
27	54012.0
28	67546.0
29	83563.0
30	102880.0
31	129480.0
32	166524.0
33	222796.0
34	342906.0
35	511205.0
36	893598.0
37	1612770.0
38	1581493.0
39	397017.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.989756383888164	20.98421938628225	13.362653963540838	23.663370266288748
2	15.40430713611443	20.797726011281547	40.397805350895574	23.400161501708453
3	18.065209666727235	27.577045646642684	29.727152028763793	24.630592657866295
4	12.425248056922324	17.34837296797344	39.10423863322426	31.122140341879973
5	12.833762300606189	38.919535806816356	34.802101151521704	13.444600741055757
6	29.83794528537238	38.25280051792024	18.099148364740618	13.810105831966753
7	26.492400505211876	32.71074821680578	23.45088175657711	17.34596952140523
8	26.092365357239096	35.296327407436344	20.127476087574657	18.483831147749903
9	26.859779087476994	15.041821077227604	21.16004499610563	36.93835483918978
10-11	25.449683457541422	26.059079915760243	27.815831843567494	20.67540478313084
12-13	25.830324804808996	23.94192619464259	28.062344850038663	22.165404150509755
14-15	23.293785153076726	25.028750333314733	25.71948697437042	25.95797753923812
16-17	22.070123422339268	27.734644877362495	26.806804991153665	23.388426709144568
18-19	21.68389844165615	27.572927727935024	29.017510158183097	21.725663672225725
20-21	23.050512700166617	26.68791080253457	28.41773174795546	21.843844749343354
22-23	23.393126730727552	26.273525569731248	28.118604001474658	22.214743698066545
24-25	22.794485901786395	26.50560896920371	28.576073953328184	22.123831175681712
26-27	22.571528013556023	26.7858167177498	28.01202895382674	22.63062631486744
28-29	22.02707860559475	27.105596143870635	28.40797928068404	22.45934596985058
30-31	22.918414487650836	26.680964207287623	28.291890903879025	22.10873040118252
32-33	23.04510470692626	26.939070480837866	27.721606089378447	22.294218722857423
34-35	22.92921181265571	26.921013833314987	28.327824605410235	21.821949748619073
36-37	22.769437251692437	26.604191271976568	28.079711436883947	22.546660039447048
38-39	22.811269180828617	26.65283383306047	28.442138868823235	22.09375811728768
40-41	22.528347706858533	26.037488320073475	28.908994477509037	22.525169495558952
42-43	22.41985574980675	26.231926478973772	28.25083075175243	23.097387019467046
44-45	22.44897864264434	26.9108634637571	28.171970444483875	22.468187449114684
46-47	22.48234684384187	26.634619394589027	27.643562750852173	23.239471010716926
48-49	23.00672458280958	26.53275642002111	27.795795013599168	22.66472398357014
50-51	22.83315808747492	26.509290097683934	27.518625621823055	23.138926193018094
52-53	22.351545397605005	26.94839010747087	27.429071087049543	23.27099340787458
54-55	22.26318436911761	26.874960708147132	27.430577545858192	23.431277376877066
56-57	23.001977302416265	26.47081440240166	27.128382956068663	23.39882533911341
58-59	22.224239432488297	26.836884415815565	27.755752976875225	23.183123174820917
60-61	22.557968196795123	27.239067919644228	27.168260795954552	23.0347030876061
62-63	22.70312870990569	26.70232904657667	27.06439452517432	23.53014771834332
64-65	21.97331747442798	26.662022376311857	27.354115692905694	24.01054445635447
66-67	22.51607244958581	26.481232736883754	27.17688903606852	23.82580577746192
68-69	22.04143876344145	26.599234545925	27.384602457347263	23.97472423328629
70-71	22.10811968110959	26.220229600723442	27.490949562969817	24.180701155197145
72-73	22.41912938213586	26.589396827345883	27.33856806688502	23.65290572363323
74-75	22.56170406930921	26.859916818588133	27.07420767693531	23.504171435167347
76-77	22.26789651077341	26.697908882597897	26.88399786671297	24.15019673991572
78-79	22.159288180145584	26.6686717639029	26.991722384985646	24.18031767096587
80-81	21.95313113467204	27.116966435609278	27.110641122426827	23.819261307291853
82-83	22.5636721587593	26.39531547730757	27.76948202982857	23.27153033410456
84-85	22.627058818696053	26.689090015682286	26.815805631717303	23.868045533904354
86-87	21.683362293711568	27.317334119666516	27.14661827120805	23.852685315413865
88-89	22.24162933555584	26.299201721943994	27.16773140947888	24.29143753302129
90-91	22.255119893952294	26.24326494973997	27.185073200807807	24.31654195549993
92-93	22.463560062625486	26.451407964983304	26.957205349304207	24.127826623087
94-95	21.602855384348253	26.37426030228192	27.390969680338962	24.631914633030867
96-97	21.834461408091858	26.280520593123125	27.134876668072543	24.750141330712474
98-99	21.374997761742595	26.76247364379236	27.010871768508604	24.851656825956436
100	21.558814432869823	26.530324017912026	26.534478789349215	25.37638275986894
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	416.0
1	300.5
2	262.0
3	354.5
4	322.5
5	324.5
6	534.5
7	1062.0
8	1738.5
9	2186.5
10	2178.5
11	2176.5
12	2506.5
13	2857.0
14	3343.5
15	4047.5
16	4775.0
17	5477.5
18	6238.0
19	6945.0
20	7749.0
21	8718.5
22	9801.5
23	11095.5
24	12610.0
25	14659.5
26	17676.0
27	21426.0
28	26447.0
29	35494.0
30	48936.0
31	63319.5
32	82465.0
33	103332.0
34	123187.0
35	143888.5
36	166130.0
37	196386.0
38	208990.5
39	203742.5
40	195668.0
41	185353.5
42	184018.0
43	193510.0
44	208398.5
45	236355.0
46	260998.5
47	276237.5
48	288908.5
49	287624.0
50	281440.0
51	281279.5
52	276840.0
53	264912.5
54	254372.0
55	227453.0
56	200575.5
57	169856.0
58	136159.5
59	120832.5
60	98953.5
61	73105.5
62	55528.5
63	43626.5
64	31279.5
65	18203.0
66	11772.5
67	8597.0
68	6612.0
69	5184.5
70	3894.0
71	2755.5
72	1975.0
73	1412.0
74	1029.5
75	703.5
76	460.5
77	284.5
78	157.5
79	85.0
80	46.0
81	33.0
82	17.0
83	8.5
84	2.5
85	1.5
86	2.0
87	0.5
88	0.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005441508665253734
2	0.0339202876341173
3	0.0013177442636654344
4	0.0012867385162850713
5	0.00666623568677808
6	4.650862107054474E-5
7	8.371551792698054E-4
8	0.006557715570946809
9	0.001255732768904708
10-11	2.8680316326835925E-4
12-13	6.976293160581711E-5
14-15	8.8366380034035E-4
16-17	6.976293160581711E-5
18-19	0.0011704669636087093
20-21	1.47277300056725E-4
22-23	0.0014727730005672502
24-25	0.0012169755846792541
26-27	0.002278922432456692
28-29	1.7053161059199737E-4
30-31	0.0019146049007374253
32-33	0.0016045474269337937
34-35	7.286350634385343E-4
36-37	0.0038912212962355767
38-39	9.844324793265304E-4
40-41	0.003844712675165032
42-43	0.0036431753171926712
44-45	0.003883469859390486
46-47	0.0023874425482879632
48-49	0.008906400935009318
50-51	0.006681738560468262
52-53	0.00460435348598393
54-55	0.004472579059617386
56-57	0.00333311784338904
58-59	0.005658548896916277
60-61	0.007766939718780972
62-63	0.0031393319222617704
64-65	0.003147083359106861
66-67	0.0038602155488552136
68-69	0.0019843678323432424
70-71	0.002728505769471958
72-73	0.006557715570946809
74-75	0.008348297482162781
76-77	0.004294296012180298
78-79	0.0029377945642894096
80-81	0.0022246623745410567
82-83	0.005046185386154104
84-85	0.0022711709956116013
86-87	0.0034881465802908554
88-89	5.115948317759921E-4
90-91	0.0017440732901454277
92-93	1.2402298952145265E-4
94-95	0.002278922432456692
96-97	0.0018680962796668804
98-99	0.0013409985742007067
100	3.100574738036316E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6450417.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.24870986059301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65458334015271	42.32035745022829
2	16.04389679318353	18.69072578678892
3	5.384346001714954	9.408936241288151
4	2.3100724048895223	5.382349490774884
5	1.1588406505303195	3.375048641370072
6	0.6567337801004549	2.2952337247633134
7	0.4094288479161232	1.6694091519576182
8	0.29529072705062953	1.3760243107596546
9	0.19103258986731342	1.001466171098892
>10	0.8275773924754146	8.01437486557072
>50	0.03980310322396008	1.6123966967337304
>100	0.025873329970985466	2.8525536593671275
>500	0.001795809812568331	0.7113041703863293
>1k	6.216249527127741E-4	0.523640340950025
>5k	3.4534719593089914E-5	0.16949765712640646
>10k+	6.906943918617983E-5	0.5966816408359208
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16165	0.25060395320178525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13497	0.2092422861963808	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8426	0.13062721371347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.550287369018158E-5	0.0	0.0	0.0	0.0
3	1.550287369018158E-5	0.0	0.0	0.0	0.0
4	1.550287369018158E-5	1.550287369018158E-5	0.0	0.0	0.0
5	1.550287369018158E-5	1.550287369018158E-5	0.0	0.0	0.0
6	1.550287369018158E-5	1.550287369018158E-5	0.0	0.0	0.0
7	1.550287369018158E-5	1.550287369018158E-5	0.0	0.0	3.100574738036316E-5
8	1.550287369018158E-5	1.550287369018158E-5	0.0	0.0	3.100574738036316E-5
9	1.550287369018158E-5	1.550287369018158E-5	0.0	0.0	3.100574738036316E-5
10-11	1.550287369018158E-5	3.100574738036316E-5	0.0	0.0	3.100574738036316E-5
12-13	1.550287369018158E-5	3.100574738036316E-5	0.0	0.0	3.100574738036316E-5
14-15	1.550287369018158E-5	3.100574738036316E-5	0.0	0.0	5.426005791563553E-5
16-17	1.550287369018158E-5	3.100574738036316E-5	0.0	0.0	7.75143684509079E-5
18-19	1.550287369018158E-5	3.100574738036316E-5	0.0	0.0	7.75143684509079E-5
20-21	1.550287369018158E-5	4.650862107054474E-5	0.0	3.100574738036316E-5	7.75143684509079E-5
22-23	1.550287369018158E-5	4.650862107054474E-5	0.0	1.1627155267636185E-4	1.0852011583127106E-4
24-25	1.550287369018158E-5	4.650862107054474E-5	0.0	3.3331178433890397E-4	1.3952586321163421E-4
26-27	1.550287369018158E-5	5.426005791563553E-5	0.0	0.0011704669636087093	1.3952586321163421E-4
28-29	1.550287369018158E-5	6.201149476072633E-5	0.0	0.003883469859390486	1.3952586321163421E-4
30-31	1.550287369018158E-5	6.201149476072633E-5	0.0	0.015030036042631041	1.550287369018158E-4
32-33	1.550287369018158E-5	6.201149476072633E-5	0.0	0.045911760433472754	1.705316105919974E-4
34-35	1.550287369018158E-5	6.201149476072633E-5	0.0	0.09491634416813673	1.705316105919974E-4
36-37	1.550287369018158E-5	6.201149476072633E-5	0.0	0.1758180905203493	1.705316105919974E-4
38-39	1.550287369018158E-5	6.201149476072633E-5	0.0	0.3007324952789874	1.86034484282179E-4
40-41	1.550287369018158E-5	6.976293160581711E-5	0.0	0.4380802047371511	2.325431053527237E-4
42-43	1.550287369018158E-5	7.75143684509079E-5	0.0	0.5770789702433192	2.325431053527237E-4
44-45	1.550287369018158E-5	7.75143684509079E-5	0.0	0.7428822043598111	2.5579741588799607E-4
46-47	1.550287369018158E-5	7.75143684509079E-5	0.0	0.9402492893095129	2.7905172642326843E-4
48-49	2.3254310535272374E-5	1.1627155267636185E-4	0.0	1.1607311589312754	3.100574738036316E-4
50-51	3.100574738036316E-5	1.2402298952145265E-4	0.0	1.3909023246094012	3.255603474938132E-4
52-53	4.650862107054474E-5	1.2402298952145265E-4	0.0	1.6161280735803591	3.6431753171926716E-4
54-55	4.650862107054474E-5	1.2402298952145265E-4	0.0	1.8652204965973518	3.953232790996303E-4
56-57	4.650862107054474E-5	1.2402298952145265E-4	0.0	2.149240894038323	4.030747159447211E-4
58-59	4.650862107054474E-5	1.2402298952145265E-4	0.0	2.475863188379914	4.1082615278981193E-4
60-61	6.201149476072633E-5	1.2402298952145265E-4	0.0	2.818228650953884	4.3408046332508424E-4
62-63	6.201149476072633E-5	1.2402298952145265E-4	0.0	3.168268036004494	4.3408046332508424E-4
64-65	7.75143684509079E-5	1.2402298952145265E-4	0.0	3.521710301830099	4.41831900170175E-4
66-67	7.75143684509079E-5	1.3177442636654345E-4	0.0	3.9140105205601436	4.4958333701526583E-4
68-69	1.0852011583127106E-4	1.3952586321163421E-4	0.0	4.351811673570872	4.4958333701526583E-4
70-71	1.0852011583127106E-4	1.3952586321163421E-4	0.0	4.827269306775051	4.728376475505382E-4
72-73	1.0852011583127106E-4	1.3952586321163421E-4	0.0	5.312067421377564	5.270977054661738E-4
74-75	1.0852011583127106E-4	1.3952586321163421E-4	0.0	5.783649336159197	5.426005791563553E-4
76-77	1.0852011583127106E-4	1.3952586321163421E-4	0.0	6.300189584642357	5.426005791563553E-4
78-79	1.0852011583127106E-4	1.3952586321163421E-4	0.0	6.860130128021181	5.426005791563553E-4
80-81	1.0852011583127106E-4	1.3952586321163421E-4	0.0	7.452068602696539	5.658548896916276E-4
82-83	1.0852011583127106E-4	1.3952586321163421E-4	0.0	8.061812127805071	6.046120739170817E-4
84-85	1.0852011583127106E-4	1.3952586321163421E-4	0.0	8.652293332353551	6.046120739170817E-4
86-87	1.0852011583127106E-4	1.3952586321163421E-4	0.0	9.2566418574179	6.201149476072632E-4
88	1.0852011583127106E-4	1.3952586321163421E-4	0.0	9.730254648652947	6.201149476072632E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10925	0.0	52.806515	1
GTATCAA	19995	0.0	41.997597	1
CAACGCA	23850	0.0	33.60126	5
ATCAACG	24190	0.0	33.14893	3
TATCAAC	26045	0.0	32.098537	2
AACGCAG	26415	0.0	30.958593	6
TCAACGC	27795	0.0	29.153692	4
GTACTAG	4615	0.0	28.73041	1
GTGGTAT	3395	0.0	28.390858	1
ACGCAGA	28585	0.0	27.934298	7
CGCAGAG	29040	0.0	27.464252	8
GTACATG	31195	0.0	26.482023	1
TACATGG	31585	0.0	25.947432	2
ACATGGG	30850	0.0	25.444132	3
GCAGAGT	31925	0.0	24.760773	9
TAGGCAT	5870	0.0	24.582151	5
GGGCACC	5770	0.0	24.272787	94
TATTCTC	3355	0.0	23.396105	5
TAGTACT	6695	0.0	23.378498	4
CTGTGCG	6745	0.0	23.20232	9
>>END_MODULE