FastQCFastQC Report
Thu 26 May 2016
ERR580176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4603971
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120910.26262111555437684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99610.21635670598272663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61650.13390614319681857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65250.046.9931871
GTATCAA115700.039.7126661
GTACTAG33400.035.2015151
CAACGCA129700.033.5375865
ATCAACG133350.032.692983
GCATCGT33450.031.8961288
TATCAAC143350.031.7581332
TAGGCAT42100.030.4937345
GTACATG165250.030.4516011
AACGCAG147950.030.0861636
TCAACGC149900.029.3654964
TACATGG168550.029.3258912
ACATGGG168150.028.0245533
CTAGGCA47400.027.9791074
ACGCAGA157400.027.5924077
CATGGGG140950.026.42554
TAGTACT45300.026.36934
CGCAGAG164800.026.2955288
GTGGTAT23000.026.1727871
CTGTGCG46200.025.3325969
Produced by FastQC (version 0.11.5)