FastQCFastQC Report
Thu 26 May 2016
ERR580176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4603971
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108300.2352317162727567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90250.19602643022729724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62890.13659947032681136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.046.5389861
GTATCAA117150.037.5189821
CAACGCA132800.031.9234625
ATCAACG136600.030.9321783
TATCAAC150400.029.125882
AACGCAG150950.028.8933266
GTACATG158500.028.3833981
TCAACGC154500.027.71324
TACATGG160250.027.5115952
TAGGCAT41900.026.697125
GTGGTAT22200.026.6808281
ACATGGG159050.026.5660823
ACGCAGA163650.025.93317
CTAGGCA45500.025.9274124
CGCAGAG170300.024.8100558
CATGGGG129200.024.5185174
GCATCGT39850.024.295268
TAGTACT42950.024.1838764
CTAGTAC45350.023.7333873
CTGTGCG45050.023.1601229
Produced by FastQC (version 0.11.5)