FastQCFastQC Report
Thu 26 May 2016
ERR580175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8218674
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285980.3479636739454564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230980.28104290302790935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141710.17242440812228346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140850.054.017561
GTATCAA266850.041.9218561
CAACGCA309400.034.9414445
ATCAACG311700.034.696373
TATCAAC337050.032.918772
AACGCAG343700.032.1625826
GTACTAG56650.031.0445231
TCAACGC354500.030.7476944
GTGGTAT48150.029.883981
ACGCAGA369200.029.2932827
GTACATG358950.029.0301381
CGCAGAG378650.028.475188
TAGGCAT68350.028.1954295
GCATCGT57150.027.9501698
TACATGG366850.027.8610172
ACATGGG360700.027.4681053
CTGTGCG70400.026.8999
TAGTACT71050.026.6605434
GCAGAGT406000.026.0758789
CTAGGCA75600.025.7398854
Produced by FastQC (version 0.11.5)