FastQCFastQC Report
Thu 26 May 2016
ERR580175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8218674
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252850.3076530350273049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210670.2563308874399934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135700.1651117929729297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141350.054.383011
GTATCAA258550.040.8419531
CAACGCA310250.032.8593945
ATCAACG312150.032.6898963
GTACTAG53450.031.22641
TATCAAC334900.031.2241062
AACGCAG344950.030.3281866
TCAACGC351850.029.1613714
GTACATG367350.027.8496741
TAGGCAT68300.027.1824235
TACATGG374450.027.0220812
ACGCAGA375750.027.0166937
ACATGGG368900.026.5907653
CGCAGAG383850.026.385538
TAGTACT74300.026.1892644
CTAGGCA72900.026.0474744
CTGTGCG74400.025.8345769
GTGGTAT48500.025.2041991
CATGGGG279450.024.6234424
GCTATTG76850.024.5233948
Produced by FastQC (version 0.11.5)