FastQCFastQC Report
Thu 26 May 2016
ERR580174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768892
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.15829585989728545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49140.13038314708938328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.046.8295251
GTATCAA83550.039.6814231
CAACGCA97950.032.496925
ATCAACG99650.031.8532453
TATCAAC104350.031.2317892
TCAACGC104800.030.555144
AACGCAG108900.029.9885526
GTACATG137500.027.0189781
TACATGG138200.026.508522
ACGCAGA122500.025.8529957
ACATGGG142050.025.0269433
CGCAGAG126700.024.810568
CATGGGG112800.023.0939944
GTGGTAT24150.021.8094861
GCAGAGT144250.021.2413569
ATGGGGG80050.020.615975
GTACTAG16500.019.9507241
TGGTATC25750.019.7243442
TGATCGC8800.019.2251749
AGAGTAC128250.019.05723210-11
Produced by FastQC (version 0.11.5)