FastQCFastQC Report
Thu 26 May 2016
ERR580174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768892
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53870.142933254654153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.12510308069321172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.048.557331
GTATCAA90450.038.8084751
CAACGCA105750.032.2206345
ATCAACG106450.031.965453
TATCAAC111200.030.8536132
TCAACGC112100.030.5639764
AACGCAG117100.029.456886
ACGCAGA130950.026.1619227
CGCAGAG135700.025.004078
GTACATG142500.024.1696411
TACATGG142350.023.7718472
GTGGTAT24750.022.8753761
ACATGGG147400.022.3834783
CATGGGG114400.022.3491384
TATCACG2859.640644E-1121.4381072
GCAGAGT154600.021.3390589
GTACTAG16150.021.3261551
TGGTATC26350.020.5118662
ATGGGGG80850.020.0546685
GAGTACT69250.019.03635212-13
Produced by FastQC (version 0.11.5)