FastQCFastQC Report
Thu 26 May 2016
ERR580173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5986316
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154860.25868998562722045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116570.19472744171874654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76930.1285097545802794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC64550.038.95380894
GGTATCA102900.036.972881
GTATCAA172400.036.3616071
GCATCGT67400.035.7731448
GTACTAG72150.035.7185971
TAGGCAT85050.034.2230955
CAACGCA176800.033.271935
ATCAACG183250.032.1305243
CTAGGCA97850.030.2766694
CTGTGCG90050.030.2737149
TAGTACT91500.030.2707084
TATCAAC210750.029.6117732
TCTAACG32250.028.5816572
GTACATG229100.028.5119741
AACGCAG215800.028.3782356
ACTGTGC100400.028.2282058
TACCCCG5050.027.933985
TACATGG231700.027.9288752
CTAACGC32750.027.8578643
ACATGGG223700.027.581973
Produced by FastQC (version 0.11.5)