FastQCFastQC Report
Thu 26 May 2016
ERR580173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5986316
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131230.21921662672000608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101810.17007120907082085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.12822577358094694No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC65170.10886495133233862No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC59050.038.9253794
GTACTAG69550.036.835671
GTATCAA173500.034.7342381
GGTATCA106750.034.3035241
TAGGCAT82100.033.08915
CTAACGC29200.030.5826153
CAACGCA187950.030.1831915
CTAGGCA94250.030.1699284
CTGTGCG86050.029.9313499
TCTAACG30450.029.7904822
TAACGCC30550.029.692474
ATCAACG193500.029.342013
GCATCGT80750.028.927238
TACCCCG3750.028.8268265
CGCCTAC31600.028.7053767
TAGTACT89600.028.3785024
GTACATG223050.028.3247591
TACATGG223700.027.9442982
ACATGGG215650.027.8102633
ACTGTGC99000.027.4400968
Produced by FastQC (version 0.11.5)