FastQCFastQC Report
Thu 26 May 2016
ERR580172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10606466
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244920.2309157451690318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186900.17621326462555956No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC142000.13388059698678145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117500.11078147990103396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC131500.041.92179594
TACCCCG7900.034.52645
GTACTAG152850.034.4938621
TATTCTC46200.033.6927455
GTATCAA361700.033.301421
GCATCGT149200.032.5409248
GGTATCA231600.032.4112661
TAGGCAT178500.031.5096345
AGGGCTA124550.030.9236375
CAACGCA366050.030.3451925
CTGTGCG200400.029.6214319
TAGTACT203550.029.3212134
ACTGTGC204650.029.3047038
ATCAACG383050.028.9781383
CCGTGTT15800.028.8545999
TCAAGAC17650.028.5136643
CTAGGCA206400.028.3238854
CTATTGA137450.028.0052599
TACTGTG225500.027.930537
GTACATG485950.027.8161371
Produced by FastQC (version 0.11.5)