FastQCFastQC Report
Thu 26 May 2016
ERR580172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10606466
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212690.20052862093745458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166970.1574228399921331No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC147950.13949038256474872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111090.10473799661451796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC130600.043.9120394
TATTCTC50650.034.9834985
GTACTAG145900.034.8947831
TACCCCG6600.033.4699675
GTATCAA367150.032.4323921
GGTATCA238550.031.8061751
AGGGCTA127150.031.1610815
CTGTGCG202850.030.2358729
TAGTACT207100.029.639064
ACTGTGC209900.029.5112728
CAACGCA374400.029.463115
ATCAACG390300.028.2871953
GTACTGT219950.028.2481756
TACTGTG229700.028.1130947
CTAACGC72250.027.9728073
CTATTGA142300.027.9086489
GCTATTG142050.027.8914628
TTCTCTG65200.027.5358647
TCTAACG75600.027.1691022
TCTCTGC66600.027.0277278
Produced by FastQC (version 0.11.5)