FastQCFastQC Report
Thu 26 May 2016
ERR580171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10009881
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175050.17487720383489075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127870.1277437763745643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125100.046.5756721
GTATCAA225400.041.3101161
CAACGCA256950.034.5400165
ATCAACG262050.033.974753
TATCAAC281250.032.396962
AACGCAG284950.031.9034946
TCAACGC288050.031.1207334
ACGCAGA312600.028.2407847
GTACATG352950.027.2607381
CGCAGAG326400.027.0034668
TACATGG360100.026.2170372
ACATGGG359800.025.5153523
GTACTAG50650.024.6043321
GTGGTAT52400.023.9621141
CATGGGG289850.023.4996874
GCAGAGT368850.023.3897089
CTAACGC23900.022.4216443
TAGTACT72200.022.3969174
TAACGCC24500.022.2566034
CTAGTAC77400.021.985083
Produced by FastQC (version 0.11.5)