FastQCFastQC Report
Thu 26 May 2016
ERR580171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10009881
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159150.1589928991163831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126100.12597552358514552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125950.045.7634471
GTATCAA216000.040.7018661
CAACGCA259100.032.7427185
ATCAACG262050.032.3573153
TATCAAC278400.031.3272912
TCAACGC287100.029.7302424
AACGCAG291750.029.5438696
GTACTAG52100.027.251831
ACGCAGA318000.026.5730537
GTACATG348850.026.0641881
CGCAGAG331300.025.3787358
TACATGG351600.025.3668562
ACATGGG353600.024.2987583
CATGGGG279950.022.296334
GCAGAGT373550.022.192329
TAGTACT76550.021.8585114
CTGTGCG75700.021.6041039
TAGGCAT70400.021.5642725
ATGGGGG197200.020.8786355
CTAACGC22550.020.6352043
Produced by FastQC (version 0.11.5)