FastQCFastQC Report
Thu 26 May 2016
ERR580170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12619973
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304130.2409910068745789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227300.18011132036494848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144360.11439010210243714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169850.046.1881261
GTATCAA304500.042.1363451
CAACGCA343500.035.4141245
ATCAACG355900.034.2531053
TATCAAC386050.032.9799272
GTACTAG93350.032.3963781
AACGCAG397400.031.3652846
TCAACGC408750.030.1097984
ACGCAGA423000.028.7554787
GTACATG456150.028.2412661
GCATCGT96300.027.9654338
CGCAGAG437300.027.7611948
TACATGG459600.027.4565282
TAGGCAT114750.027.3735145
ACATGGG451700.026.832263
TAGTACT129900.025.9942464
CTAGGCA127800.025.7958054
CTGTGCG130450.025.581229
GGGCACC117500.025.3195394
TCTAACG42950.024.9675922
Produced by FastQC (version 0.11.5)