FastQCFastQC Report
Thu 26 May 2016
ERR580170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12619973
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258180.20458046938769203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206310.16347895514514968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138700.10990514797456381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172750.045.7153361
GTATCAA303350.039.9183431
CAACGCA353400.032.9823655
ATCAACG366950.031.828753
TATCAAC393400.030.5613942
GTACTAG90200.030.4873711
AACGCAG408050.029.4048256
TCAACGC415450.028.3956384
GTACATG456950.027.456841
CTGTGCG131200.027.2604929
TAGTACT131200.027.189714
ACGCAGA435100.026.7774127
TACATGG463600.026.5825252
GGGCACC118750.026.32378694
ACATGGG455100.025.8496153
CGCAGAG452000.025.6930398
CTAACGC39800.025.2714863
TAGGCAT117900.025.074575
CTAGTAC146750.025.0134183
TAACGCC40250.024.871984
Produced by FastQC (version 0.11.5)