FastQCFastQC Report
Thu 26 May 2016
ERR580169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8617136
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223740.25964543207859314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178090.20666959416678582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107610.12487907815311258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130100.045.7325021
GTATCAA223800.039.8464851
CAACGCA250950.033.8256575
ATCAACG259600.032.7578623
TATCAAC281050.031.3788472
GTACTAG63600.030.542511
AACGCAG285450.030.429596
GTGGTAT41750.029.0653131
TCAACGC297900.028.9080964
GTACATG319700.028.2321591
ACGCAGA309400.027.4813087
TACATGG324000.027.335352
TAGTACT85900.027.267034
CTGTGCG87200.026.6265249
CGCAGAG319800.026.5569788
ACATGGG321550.026.4320893
TAGGCAT81750.025.5993235
CTAGTAC96800.025.3153673
GCATCGT71450.024.7334428
GGGCACC82500.024.61068594
Produced by FastQC (version 0.11.5)