FastQCFastQC Report
Thu 26 May 2016
ERR580169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8617136
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196610.22816165370953875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163570.18981944813218685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104840.12166455304871596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131050.045.338821
GTATCAA221750.039.6192131
CAACGCA258150.032.5720755
ATCAACG264850.031.8724983
GTACTAG60650.031.5445081
TATCAAC287250.030.5326062
AACGCAG295300.029.2525776
TCAACGC297650.028.5811654
GTACATG310550.027.4729481
TAGTACT92550.027.0173324
CTGTGCG93400.026.7195849
GGGCACC85550.026.3744894
GTGGTAT43900.026.2338311
ACGCAGA321000.026.2224087
TACATGG320300.026.2081762
TAGGCAT80000.025.3805485
CGCAGAG331350.025.3749628
ACATGGG312500.025.3280543
CTAGTAC104200.024.5381033
CATGGGG259150.023.6864094
Produced by FastQC (version 0.11.5)