FastQCFastQC Report
Thu 26 May 2016
ERR580168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10881668
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310030.28491036484480137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259560.23852960777704302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148590.13655075674060263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186400.050.5772131
GTATCAA332050.040.102941
CAACGCA379950.033.681875
ATCAACG385300.033.2286833
TATCAAC415400.031.7733042
AACGCAG423200.030.8575766
GTACTAG75550.030.4963671
TCAACGC432150.029.8218824
GCATCGT71750.028.4231858
ACGCAGA459700.027.609537
GTACATG481300.027.4717241
CGCAGAG467100.027.1618168
TACATGG489550.026.6149672
TAGGCAT94400.026.545435
TAGTACT97900.026.4143854
GTGGTAT66400.026.059511
CTGTGCG98350.026.0441939
ACATGGG486550.025.8016993
CTAGGCA105350.025.0820054
GCAGAGT512800.024.1685669
Produced by FastQC (version 0.11.5)