FastQCFastQC Report
Thu 26 May 2016
ERR580168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10881668
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276150.25377543222233945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245140.2252779629005406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138990.12772857984639854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186550.050.7421881
GTATCAA328550.039.1328471
GTACTAG71100.032.570841
CAACGCA381400.032.506175
ATCAACG386400.032.0623933
TATCAAC418000.030.559682
AACGCAG425600.029.659796
TCAACGC433350.028.9569034
GTGGTAT68750.027.6470891
ACGCAGA464600.026.5631287
TAGGCAT91800.026.0583425
CGCAGAG475850.025.8759848
GTACATG479600.025.7557011
TAGTACT100200.025.7504164
CTGTGCG101550.025.684959
TACATGG485500.025.0524792
CTAGGCA99000.024.7807794
ACATGGG481000.024.544953
GCATCGT85950.024.0587188
CTAGTAC115700.023.316653
Produced by FastQC (version 0.11.5)