##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580168_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10881668
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51850286187742	34.0	31.0	34.0	31.0	34.0
2	32.72144849484472	34.0	31.0	34.0	31.0	34.0
3	32.79901316599624	34.0	31.0	34.0	31.0	34.0
4	36.27437870738199	37.0	37.0	37.0	35.0	37.0
5	36.20950133747878	37.0	37.0	37.0	35.0	37.0
6	36.23853374317246	37.0	37.0	37.0	35.0	37.0
7	36.20160282412586	37.0	37.0	37.0	35.0	37.0
8	36.19482242979661	37.0	37.0	37.0	35.0	37.0
9	37.981875756547616	39.0	38.0	39.0	35.0	39.0
10-11	37.93069720561223	39.0	38.0	39.0	35.0	39.0
12-13	37.88463822825692	39.0	38.0	39.0	35.0	39.0
14-15	39.27514196352986	41.0	39.0	41.0	36.0	41.0
16-17	39.261674496961305	41.0	39.0	41.0	36.0	41.0
18-19	39.26747126451569	41.0	39.0	41.0	36.0	41.0
20-21	39.25432148821302	41.0	39.0	41.0	36.0	41.0
22-23	39.18047743232012	41.0	39.0	41.0	35.5	41.0
24-25	39.097769845578824	40.5	39.0	41.0	35.0	41.0
26-27	38.958775851275746	40.0	38.0	41.0	35.0	41.0
28-29	38.852792513059576	40.0	38.0	41.0	35.0	41.0
30-31	38.73272558030625	40.0	38.0	41.0	35.0	41.0
32-33	38.585127160652206	40.0	38.0	41.0	34.5	41.0
34-35	38.55039746663839	40.0	38.0	41.0	34.5	41.0
36-37	38.588838218552524	40.0	38.0	41.0	35.0	41.0
38-39	38.490455966860964	40.0	38.0	41.0	34.0	41.0
40-41	38.35925705507648	40.0	38.0	41.0	34.0	41.0
42-43	38.32011452655971	40.0	38.0	41.0	34.0	41.0
44-45	38.20510688251103	40.0	38.0	41.0	34.0	41.0
46-47	37.98397906460664	40.0	37.0	41.0	33.0	41.0
48-49	37.74571586819226	40.0	37.0	41.0	33.0	41.0
50-51	37.559156463880356	40.0	36.5	41.0	33.0	41.0
52-53	37.37452282131747	40.0	36.0	41.0	32.5	41.0
54-55	37.166584847102484	39.0	35.5	41.0	32.0	41.0
56-57	36.939663386164696	39.0	35.0	41.0	32.0	41.0
58-59	36.69271549177938	39.0	35.0	41.0	31.0	41.0
60-61	36.43190065162804	38.0	35.0	40.0	31.0	41.0
62-63	36.14035918022862	37.5	35.0	40.0	31.0	41.0
64-65	35.84870995880411	37.0	35.0	40.0	30.5	41.0
66-67	35.53019238410876	36.5	35.0	39.5	30.0	41.0
68-69	35.19132218516499	36.0	34.5	39.0	30.0	41.0
70-71	34.88877596706682	35.5	34.0	39.0	29.0	40.5
72-73	34.55908487559077	35.0	34.0	38.0	29.0	40.0
74-75	34.21995538735422	35.0	34.0	37.0	29.0	39.5
76-77	33.00510464020773	34.5	32.5	36.0	27.0	39.0
78-79	33.64220586402746	35.0	33.5	36.0	29.0	39.0
80-81	33.58986774821654	35.0	34.0	36.0	29.0	38.0
82-83	33.38713398534122	35.0	34.0	36.0	29.0	37.0
84-85	33.17249892204026	35.0	34.0	35.0	29.0	37.0
86-87	32.99804818525983	35.0	33.0	35.0	29.0	36.5
88-89	32.83582742094319	35.0	33.0	35.0	28.5	36.0
90-91	32.66988006801898	35.0	33.0	35.0	27.0	36.0
92-93	32.54400538593899	35.0	33.0	35.0	27.0	35.5
94-95	32.44513607656474	35.0	33.0	35.0	27.0	35.0
96-97	32.34445734789924	35.0	33.0	35.0	27.0	35.0
98-99	32.2700871318625	35.0	33.0	35.0	27.0	35.0
100	30.997754388389723	34.0	30.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1183331550770248
1101	2	0.0604983256612428
1101	3	0.0018581545529414711
1101	4	0.013348195202574686
1101	5	0.007389352141892402
1101	6	0.037155286554970246
1101	7	0.024107190548029678
1101	8	-0.013263358577603412
1101	9	-0.0047967188655917425
1101	10-11	0.016269070407510355
1101	12-13	-0.025518542748301343
1101	14-15	-0.0025255386040612393
1101	16-17	-0.031153809123161125
1101	18-19	-0.04318465234990754
1101	20-21	-0.06805107311549818
1101	22-23	-0.07616558784971517
1101	24-25	-0.131285472337197
1101	26-27	-0.11679437013418692
1101	28-29	-0.10935754463782388
1101	30-31	-0.12762749830590536
1101	32-33	-0.08747231917148213
1101	34-35	-0.12019503224691874
1101	36-37	-0.0037010123405778472
1101	38-39	-0.02064110316951684
1101	40-41	0.007651445482949271
1101	42-43	-0.00938309289368533
1101	44-45	-0.06350807439532247
1101	46-47	0.013574777180210162
1101	48-49	-0.030263018021493338
1101	50-51	0.020759949624768126
1101	52-53	0.022004729539666812
1101	54-55	0.0280952603660225
1101	56-57	-0.002487286988113624
1101	58-59	0.010537644124553935
1101	60-61	-0.0843697842582074
1101	62-63	-0.041835153674647074
1101	64-65	-0.04836724523949698
1101	66-67	-0.06448499026780041
1101	68-69	-0.036319622343725655
1101	70-71	-0.06124372926073107
1101	72-73	-0.029402726524835998
1101	74-75	0.001959665346639383
1101	76-77	-0.021175084776658082
1101	78-79	0.0028473799799471067
1101	80-81	0.018624954750009692
1101	82-83	0.040780104892100155
1101	84-85	-0.0039375081071000295
1101	86-87	-0.039407228684247286
1101	88-89	0.023123073002999206
1101	90-91	-0.05777877218978489
1101	92-93	-0.08656083904304523
1101	94-95	-0.11121502989383458
1101	96-97	-0.07348391577605895
1101	98-99	-0.1321670875794183
1101	100	-0.05564885847335077
1102	1	0.1241112745907671
1102	2	0.049388747306039704
1102	3	0.019033344239943517
1102	4	0.020790732573509274
1102	5	0.018682288889714016
1102	6	0.020747015187595252
1102	7	0.023818210142991347
1102	8	0.04003473696988635
1102	9	0.046708982633980156
1102	10-11	0.007535192290809789
1102	12-13	-0.0123960903147875
1102	14-15	0.04704920761934517
1102	16-17	-0.011739471679263147
1102	18-19	-0.03013363517558787
1102	20-21	0.012999840534362761
1102	22-23	-0.02975368720579752
1102	24-25	-0.029455402660332197
1102	26-27	-0.0645784942356542
1102	28-29	-0.06396400664463897
1102	30-31	-0.050699535004987695
1102	32-33	-0.03437277237009795
1102	34-35	-0.06645774010673478
1102	36-37	0.005776211982627899
1102	38-39	-0.021828538852624035
1102	40-41	-0.006121527917947844
1102	42-43	0.05158588165512157
1102	44-45	0.05973761104949915
1102	46-47	0.07778302694616457
1102	48-49	0.053605778091394995
1102	50-51	0.06962705881338138
1102	52-53	0.08678940078142006
1102	54-55	0.04612823561242152
1102	56-57	0.015462424653136964
1102	58-59	0.02650808907588953
1102	60-61	0.06910579568386055
1102	62-63	0.08502428387799199
1102	64-65	0.013726475181705666
1102	66-67	0.03284575337528395
1102	68-69	0.03958267069764787
1102	70-71	0.021054480721893754
1102	72-73	0.06641993049634465
1102	74-75	-0.011953121048826176
1102	76-77	-0.02129070400388855
1102	78-79	0.08489383268986472
1102	80-81	0.11604043366946115
1102	82-83	0.08310474744303775
1102	84-85	0.06991986817546092
1102	86-87	0.05040719526684967
1102	88-89	0.12455934799001511
1102	90-91	0.10226663986251339
1102	92-93	0.05685905675079539
1102	94-95	0.06942238643340914
1102	96-97	0.011564830795649073
1102	98-99	-0.019108352589086053
1102	100	0.06133070889421788
1103	1	0.11460729874864484
1103	2	0.06001340622117368
1103	3	0.02964939808700251
1103	4	8.600228514623609E-4
1103	5	-0.029303136360930182
1103	6	0.008920111382977325
1103	7	0.0047279767300949516
1103	8	-0.0324295491391382
1103	9	-0.013478471490145694
1103	10-11	-0.045688089618629135
1103	12-13	-0.04485592313169917
1103	14-15	-0.008729186638532838
1103	16-17	-0.018135163114905595
1103	18-19	-0.03169552702181733
1103	20-21	-0.054178676642173684
1103	22-23	-0.11273891829188898
1103	24-25	-0.11928066769071677
1103	26-27	-0.12280889671188078
1103	28-29	-0.1185867173926809
1103	30-31	-0.15651238553076752
1103	32-33	-0.14954692908083445
1103	34-35	-0.14076277816658944
1103	36-37	-0.12251972071252482
1103	38-39	-0.14168226031799946
1103	40-41	-0.1327636825626186
1103	42-43	-0.16027208424315376
1103	44-45	-0.14722366457731084
1103	46-47	-0.12509199320608388
1103	48-49	-0.15589151527168355
1103	50-51	-0.14094322555744299
1103	52-53	-0.14284523827641493
1103	54-55	-0.10818660680451586
1103	56-57	-0.08989517457690965
1103	58-59	-0.09739417716958343
1103	60-61	-0.14392579224212199
1103	62-63	-0.14254254715874737
1103	64-65	-0.12911238251074764
1103	66-67	-0.10055220972210321
1103	68-69	-0.06982810615917856
1103	70-71	-0.06993950669853177
1103	72-73	-0.10402856336477129
1103	74-75	-0.18499293460681088
1103	76-77	-0.1999940837078995
1103	78-79	-0.0685938390891252
1103	80-81	-0.03638623575715627
1103	82-83	-0.028973121986140882
1103	84-85	-0.006733853764210096
1103	86-87	-0.06421516992335086
1103	88-89	-0.05851059982185092
1103	90-91	-0.025601478660952637
1103	92-93	-0.07875329695934141
1103	94-95	-0.07813656283526882
1103	96-97	-0.13223805284227552
1103	98-99	-0.08476644486159302
1103	100	-0.04109998145371918
1104	1	0.15636161807707083
1104	2	0.10285573434706663
1104	3	0.07467242864025536
1104	4	0.029135648142684545
1104	5	0.011986281756847461
1104	6	0.046434052076115506
1104	7	0.006892593184190332
1104	8	0.022672620588345183
1104	9	0.015563915762484726
1104	10-11	0.025356635307332454
1104	12-13	0.035511021301026346
1104	14-15	0.03745253149365624
1104	16-17	0.025836620064815463
1104	18-19	-0.008293093432968135
1104	20-21	0.001590376907088853
1104	22-23	-0.02664582757063272
1104	24-25	0.020077885552112207
1104	26-27	0.01818328247033918
1104	28-29	-0.04820911674892869
1104	30-31	-0.06848658999771118
1104	32-33	-0.046705251524763014
1104	34-35	-0.048376722038788955
1104	36-37	-0.06602559191863833
1104	38-39	0.00865257750711379
1104	40-41	-0.0017436501031156126
1104	42-43	0.041935490051280055
1104	44-45	-0.00409039674982381
1104	46-47	0.03375335264838952
1104	48-49	0.015650684131607306
1104	50-51	0.022083523940160887
1104	52-53	-0.015883696870034214
1104	54-55	-0.0519695506082698
1104	56-57	0.04544079084175934
1104	58-59	-0.0016006268389006095
1104	60-61	-0.03867596571773646
1104	62-63	-0.01486846347092552
1104	64-65	-0.045025502865684075
1104	66-67	-0.025075040469317855
1104	68-69	0.02238180026970582
1104	70-71	-0.037955119313004104
1104	72-73	-0.04934243163123142
1104	74-75	-0.09461267082239289
1104	76-77	-0.17122416713787914
1104	78-79	-0.050039187169112154
1104	80-81	-0.019164273554643785
1104	82-83	0.03528539930529462
1104	84-85	0.014794298604378753
1104	86-87	0.0188434221729068
1104	88-89	0.0961929022752841
1104	90-91	0.07588879472314858
1104	92-93	0.011334127486122725
1104	94-95	0.019080761963564896
1104	96-97	-0.0025585789603965736
1104	98-99	0.006976893717151711
1104	100	0.0017418325843685523
1105	1	0.1369402361981784
1105	2	0.08855291955368472
1105	3	0.04189736380231324
1105	4	0.038033208624213444
1105	5	0.018245405012180527
1105	6	0.026087580747812922
1105	7	0.017140971420090523
1105	8	-0.015344817474868933
1105	9	0.008204849349176868
1105	10-11	0.030696202099825598
1105	12-13	-8.116350493097002E-4
1105	14-15	0.08041307580288759
1105	16-17	0.03710940222806158
1105	18-19	-0.01593180840133357
1105	20-21	-0.007200486384363103
1105	22-23	-0.022428858014976072
1105	24-25	-0.02680008205329898
1105	26-27	-0.019123569549464037
1105	28-29	0.003567689162430554
1105	30-31	0.004194292480541151
1105	32-33	-0.026958848172462524
1105	34-35	-0.004279037683112108
1105	36-37	0.01462332548838674
1105	38-39	-0.010230481611621656
1105	40-41	-0.013605057848060653
1105	42-43	0.016041137212461365
1105	44-45	0.013613431958695799
1105	46-47	0.02967429895537066
1105	48-49	0.006148894803416738
1105	50-51	-0.0025181459548520024
1105	52-53	-0.04459769932824997
1105	54-55	0.02659367371614252
1105	56-57	0.039719265837007356
1105	58-59	-0.021898013655082593
1105	60-61	-0.05023634580371805
1105	62-63	0.016401147364142332
1105	64-65	0.06852188886016819
1105	66-67	0.06460000346550032
1105	68-69	0.05956296809140582
1105	70-71	-0.0057275651500035
1105	72-73	-0.03366839138907096
1105	74-75	-0.07572510679213451
1105	76-77	-0.03503254421391233
1105	78-79	-0.010508232404227158
1105	80-81	-0.04961781946561672
1105	82-83	-0.0033544693899330014
1105	84-85	0.06300516955410274
1105	86-87	0.04767022146330646
1105	88-89	0.008665956387858387
1105	90-91	0.00791768188339148
1105	92-93	0.02631134736688523
1105	94-95	-0.03955906415153976
1105	96-97	-0.03154805696039631
1105	98-99	-0.06199425686185833
1105	100	0.04909429729876891
1106	1	0.11410123306995246
1106	2	0.033529142451776295
1106	3	-0.004930212588753591
1106	4	0.018515412894302585
1106	5	-0.01021569815247858
1106	6	-6.449195373505745E-4
1106	7	0.010697882526741864
1106	8	0.009560882670186288
1106	9	0.012783740773976149
1106	10-11	-0.00662773681356299
1106	12-13	0.004473085525582121
1106	14-15	0.02658220968245928
1106	16-17	-0.023627763638295107
1106	18-19	-0.014043768217980812
1106	20-21	-0.03565301343958538
1106	22-23	-0.053265220476731656
1106	24-25	-0.01525766432934006
1106	26-27	-0.06841416903930053
1106	28-29	-0.018035889869302935
1106	30-31	-0.0136992712987265
1106	32-33	-0.010524046691827493
1106	34-35	-0.01472473494119697
1106	36-37	-0.021365098378957725
1106	38-39	-0.036709514255541364
1106	40-41	-0.006942947071294725
1106	42-43	0.01775513970979148
1106	44-45	0.012073459071963555
1106	46-47	-0.02739177928144443
1106	48-49	-0.04881411466805474
1106	50-51	-0.04747374419803663
1106	52-53	-0.0012241671643025143
1106	54-55	-0.012983767159077786
1106	56-57	-0.06276085699032308
1106	58-59	-0.09510724104816148
1106	60-61	-0.060633941747433084
1106	62-63	-0.11519485085522518
1106	64-65	-0.10257758330937605
1106	66-67	-0.08887437671980791
1106	68-69	-0.06439023477658878
1106	70-71	-0.09001212320681162
1106	72-73	-0.06398831782188097
1106	74-75	-0.08279132433015235
1106	76-77	-0.07280095493849359
1106	78-79	0.012393266497007005
1106	80-81	-0.018627446390730995
1106	82-83	0.00660794552781141
1106	84-85	0.04430373142049859
1106	86-87	0.01331402422490413
1106	88-89	0.002919260501585086
1106	90-91	0.015608687567485902
1106	92-93	-0.049741231774873995
1106	94-95	-0.015252060429780556
1106	96-97	0.01639433694104042
1106	98-99	0.043107958935912905
1106	100	0.1441297595516744
1107	1	0.10787874643804685
1107	2	0.028271042188293904
1107	3	0.02295395311410431
1107	4	0.006737413505355505
1107	5	0.0184481821290845
1107	6	0.03127452603587244
1107	7	0.021211574298767744
1107	8	0.01596166837028079
1107	9	-0.003450928646138607
1107	10-11	-0.02360177503174299
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2316	10-11	-0.05102272035881583
2316	12-13	-0.04017066258591484
2316	14-15	-0.04167444775847429
2316	16-17	-0.03620599007768277
2316	18-19	-0.002088395396462772
2316	20-21	-0.017260676942093767
2316	22-23	-0.027717077899140463
2316	24-25	-0.040775706633830566
2316	26-27	-0.03529301399199625
2316	28-29	-0.023229760534668742
2316	30-31	-0.39274021365545053
2316	32-33	-0.19673690138126432
2316	34-35	-0.2943546734180913
2316	36-37	-0.5681361113718921
2316	38-39	-0.38167916008982417
2316	40-41	-0.2687518209561617
2316	42-43	-0.42653246753087615
2316	44-45	-0.27887795090089895
2316	46-47	-0.4370189857579305
2316	48-49	-0.4111629013413065
2316	50-51	-0.39458270660745853
2316	52-53	-0.4042007154024603
2316	54-55	-0.30944740790199177
2316	56-57	-1.0826936826027804
2316	58-59	-0.8801912717817189
2316	60-61	-0.529272152489888
2316	62-63	-0.2668450659881998
2316	64-65	-0.19174292532589732
2316	66-67	-0.13293329525309616
2316	68-69	-0.18863238075808653
2316	70-71	-0.23797575574406693
2316	72-73	-0.14327024385320897
2316	74-75	-0.1888973664917799
2316	76-77	-0.24903756741243654
2316	78-79	-0.2552002311489403
2316	80-81	-0.24642873438894242
2316	82-83	-0.3761039597190745
2316	84-85	-0.2304023738763945
2316	86-87	-0.2772567669257384
2316	88-89	-0.3085083168705012
2316	90-91	-0.2693357852481384
2316	92-93	-0.26620875717138404
2316	94-95	-0.19898042815123773
2316	96-97	-0.39677758890687187
2316	98-99	-0.21298335359238507
2316	100	-0.3943131392262629
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	21.0
13	108.0
14	407.0
15	1228.0
16	3122.0
17	7166.0
18	13353.0
19	20900.0
20	31647.0
21	47782.0
22	63999.0
23	67365.0
24	63881.0
25	64051.0
26	70739.0
27	85915.0
28	106110.0
29	130094.0
30	159810.0
31	200089.0
32	251961.0
33	331396.0
34	498558.0
35	763584.0
36	1368087.0
37	2743314.0
38	3042291.0
39	744508.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13584461389287	20.058928631044132	13.370587108003976	23.43463964705902
2	15.852471817357157	19.992502946944704	40.23803832686876	23.916986908829383
3	18.810696775897544	25.81159472632638	29.755656577659202	25.622051920116874
4	12.793330574531888	16.472231817986742	38.56385361737041	32.170583990110956
5	13.218623252956874	38.33066312440086	34.661195931945194	13.789517690697076
6	31.02320342800387	37.38047328773494	17.436499624873687	14.159823659387513
7	27.485656831250303	32.46468530861235	22.264974069675574	17.78468379046177
8	26.56923769833528	35.18811553813089	19.627488033280287	18.615158730253544
9	26.542685136920486	15.308566435494415	20.741788336596407	37.4069600909887
10-11	25.653643438008856	25.955936498285787	27.45290492929433	20.93751513441103
12-13	25.759796479656057	23.608558396951054	28.009911903179514	22.62173322021338
14-15	23.272382070909316	25.032511169037402	25.46041062725351	26.234696132799773
16-17	22.323645152902433	27.95256496445878	26.495544820981614	23.228245061657173
18-19	21.95561193232527	27.52719384981028	28.659095962473362	21.858098255391088
20-21	23.147405562302392	26.64009439749229	28.34614619388888	21.866353846316436
22-23	23.367675993786055	26.263577265426203	28.083369299827588	22.285377440960154
24-25	22.871553331713486	26.390103980382417	28.461721125842104	22.276621562061994
26-27	22.70350687355893	26.637410134288697	27.97580780750203	22.68327518465035
28-29	22.22644795409961	26.941836257474943	28.33741535662724	22.494300431798212
30-31	23.02692934880503	26.52172702349207	28.258451573012312	22.192892054690585
32-33	23.027506303341156	26.781046296038863	27.746534569744835	22.444912830875147
34-35	22.986788970386712	26.809014587075136	28.220977727583797	21.983218714954358
36-37	22.847984750406468	26.493121966418176	28.006620227953483	22.652273055221876
38-39	22.86500845703639	26.58268544153561	28.298209725862982	22.254096375565023
40-41	22.698391755965687	26.024427861046323	28.656296689060184	22.620883693927805
42-43	22.587500422756193	26.209105690518943	28.157639535276985	23.045754351447883
44-45	22.60585837272734	26.72699677986164	28.088915886751114	22.578228960659906
46-47	22.64952779776413	26.596329927009716	27.59564644436004	23.158495830866123
48-49	23.08523414659836	26.61406180105896	27.610858855992838	22.689845196349843
50-51	22.88153155665943	26.546301722712673	27.533116771582435	23.039049949045463
52-53	22.583315829661853	26.902995257672963	27.367941174429806	23.145747738235382
54-55	22.45182781225798	26.817939655677087	27.398408234150097	23.33182429791484
56-57	23.072569943501165	26.595118015299164	27.067560588293677	23.264751452905998
58-59	22.411966944041406	26.883332551432996	27.621068153664968	23.08363235086063
60-61	22.63801011429354	27.161201952281044	27.216349940220354	22.984437993205063
62-63	22.748685898313646	26.765290842927374	27.058203323084207	23.42781993567477
64-65	22.247740746739634	26.739068453621982	27.285300580909812	23.72789021872857
66-67	22.63488994216208	26.62052026973968	27.15252733106383	23.592062457034412
68-69	22.289015511557736	26.656221720922822	27.399111096734007	23.65565167078543
70-71	22.30383875867263	26.3460100339312	27.416121421733198	23.93402978566297
72-73	22.603246970912906	26.591383577760986	27.28039132931071	23.524978122015398
74-75	22.681310290711494	26.844097808051636	27.075302766647592	23.399289134589278
76-77	22.467770437390357	26.758269285618212	26.818648236596893	23.95531204039453
78-79	22.400475412637483	26.77228298006308	26.912658918313102	23.914582688986332
80-81	22.184161631726727	27.206637133090222	26.97409376135515	23.6351074738279
82-83	22.706582133503417	26.568001450883376	27.566145435803303	23.159270979809904
84-85	22.791568087597533	26.781158303315628	26.818616396728995	23.608657212357844
86-87	21.93053575324556	27.297405443497507	27.134739052781658	23.63731975047527
88-89	22.40405591364568	26.53282720185298	27.129431859264912	23.93368502523642
90-91	22.407082973859467	26.53005832574563	27.09043321027595	23.972425490118958
92-93	22.559344198025713	26.635715231294192	26.97587193183853	23.829068638841562
94-95	21.896877194410944	26.56869739444702	27.342862370681104	24.191563040460927
96-97	22.058852585761898	26.532682601551095	27.156181601182855	24.252283211504157
98-99	21.64887278892222	26.850992762183157	27.03761297429076	24.462521474603864
100	22.055615147429233	26.586042415036854	26.601555090583734	24.75678734695018
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1197.0
1	858.0
2	730.5
3	967.0
4	795.0
5	736.5
6	1295.0
7	2522.5
8	4198.5
9	5377.0
10	4972.5
11	4225.5
12	4507.0
13	5252.5
14	6184.0
15	7238.0
16	8350.0
17	9493.5
18	10767.0
19	11942.0
20	12991.0
21	14256.0
22	15985.0
23	18549.0
24	21604.0
25	25427.0
26	31623.0
27	39234.5
28	48777.0
29	64584.5
30	86172.0
31	108290.5
32	137997.0
33	170780.5
34	199521.0
35	229801.0
36	264800.0
37	308724.0
38	331355.5
39	331166.0
40	323660.5
41	313107.5
42	315744.5
43	332359.5
44	357479.0
45	400007.0
46	436619.5
47	458734.5
48	478318.5
49	479297.5
50	466748.0
51	466585.0
52	462626.0
53	443239.5
54	426719.0
55	385034.5
56	340655.5
57	293059.0
58	242576.5
59	218315.0
60	181136.5
61	136430.0
62	105448.5
63	82756.0
64	60117.0
65	37109.5
66	24292.0
67	17232.5
68	12491.0
69	8958.5
70	6509.5
71	4791.0
72	3502.0
73	2458.5
74	1782.5
75	1206.5
76	749.0
77	462.5
78	265.5
79	152.5
80	105.0
81	66.5
82	30.5
83	22.0
84	16.0
85	11.0
86	13.0
87	8.5
88	2.0
89	3.0
90	3.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35272165995139715
2	5.421962882896262E-4
3	0.002030938639186566
4	0.0012038595553549327
5	0.0045673144962702404
6	0.0
7	6.43283731869048E-5
8	2.84882795542007E-4
9	0.0029039665610088453
10-11	8.270790838316332E-5
12-13	3.675907039251703E-5
14-15	2.1136465475697291E-4
16-17	5.973348938784017E-5
18-19	0.0010797976927801878
20-21	2.1136465475697291E-4
22-23	1.1487209497661572E-4
24-25	0.0
26-27	0.002053913058181889
28-29	0.011381527170283085
30-31	0.003492111687289118
32-33	0.014018990470946182
34-35	0.011156377864128918
36-37	0.012663499750222115
38-39	0.012672689517820248
40-41	0.013871954189376113
42-43	0.006359319177905445
44-45	0.00136008560452313
46-47	0.005798743354419561
48-49	0.012580791841838954
50-51	0.0036667172716535737
52-53	0.005674681491844817
54-55	0.005008423340980446
56-57	2.573134927476192E-4
58-59	5.467911720886908E-4
60-61	3.492111687289118E-4
62-63	8.270790838316331E-4
64-65	2.8028791174294237E-4
66-67	0.0012819725799390314
68-69	0.0015530707240838447
70-71	8.316739676306978E-4
72-73	0.002715576325247196
74-75	0.007774543388017351
76-77	0.0036069837822657338
78-79	0.0015944246782754262
80-81	3.859702391214288E-4
82-83	1.9758000335977902E-4
84-85	0.0016036144458735555
86-87	0.0046132633342608875
88-89	0.0015990195620744908
90-91	2.205544223551022E-4
92-93	0.0019528256146024673
94-95	0.003041813074980784
96-97	0.00160820932967262
98-99	0.001929851195607144
100	0.002242303293943539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0881668E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.51300929396912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.31155402030402	33.82310609092786
2	15.933133132894085	15.77794737782064
3	6.510564168700996	9.670728725816241
4	3.1999405723866343	6.337547492029132
5	1.76460705117088	4.368550266241361
6	1.0976840909871652	3.260978555933373
7	0.733921541364853	2.5437064869049477
8	0.4985527498982197	1.974787755139553
9	0.34949762743752083	1.5574211347980713
>10	1.49522685195361	12.467877256328167
>50	0.06610554595294278	2.2507534651866967
>100	0.03547023645810394	3.3069414895466567
>500	0.0024005234998383867	0.7880277102524689
>1k	0.0012128594115184972	0.8666188221465474
>5k	5.161103182813683E-5	0.16136183794769993
>10k+	7.741654774220525E-5	0.8436455329804996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27615	0.25377543222233945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24514	0.2252779629005406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13899	0.12772857984639854	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8379535196258515E-5
2	0.0	0.0	0.0	0.0	1.8379535196258515E-5
3	0.0	0.0	0.0	0.0	1.8379535196258515E-5
4	0.0	0.0	0.0	0.0	1.8379535196258515E-5
5	0.0	0.0	0.0	0.0	1.8379535196258515E-5
6	0.0	0.0	0.0	0.0	3.675907039251703E-5
7	0.0	0.0	0.0	0.0	5.5138605588775544E-5
8	0.0	0.0	0.0	0.0	5.5138605588775544E-5
9	0.0	0.0	0.0	0.0	5.5138605588775544E-5
10-11	0.0	0.0	0.0	4.594883799064629E-6	5.5138605588775544E-5
12-13	0.0	4.594883799064629E-6	0.0	9.189767598129257E-6	7.351814078503406E-5
14-15	0.0	1.3784651397193886E-5	0.0	1.3784651397193886E-5	1.1027721117755109E-4
16-17	0.0	1.8379535196258515E-5	0.0	2.2974418995323145E-5	1.3325163017287423E-4
18-19	0.0	1.8379535196258515E-5	0.0	2.7569302794387772E-5	1.60820932967262E-4
20-21	0.0	1.8379535196258515E-5	0.0	3.21641865934524E-5	1.929851195607144E-4
22-23	0.0	1.8379535196258515E-5	0.0	1.1946697877568035E-4	1.9758000335977902E-4
24-25	0.0	2.7569302794387772E-5	0.0	4.916525664999153E-4	2.1136465475697291E-4
26-27	0.0	3.675907039251703E-5	0.0	0.0013463009531259362	2.389339575513607E-4
28-29	0.0	3.675907039251703E-5	0.0	0.004627047985658081	2.4352884135042533E-4
30-31	0.0	3.675907039251703E-5	0.0	0.017465153320244654	2.5271860894855453E-4
32-33	0.0	3.675907039251703E-5	0.0	0.04620155659959484	2.84882795542007E-4
34-35	0.0	4.594883799064629E-5	0.0	0.09197578900587666	2.84882795542007E-4
36-37	0.0	6.43283731869048E-5	0.0	0.16565475072387797	2.84882795542007E-4
38-39	0.0	8.270790838316332E-5	0.0	0.27052378366993	3.262367497335886E-4
40-41	0.0	9.189767598129258E-5	0.0	0.3837646948978778	3.308316335326533E-4
42-43	0.0	1.0108744357942183E-4	0.0	0.4965277382107228	3.492111687289118E-4
44-45	0.0	1.0108744357942183E-4	0.0	0.6339423331055496	3.813753553223642E-4
46-47	0.0	1.0108744357942183E-4	0.0	0.7991697596361146	4.181344257148812E-4
48-49	0.0	1.0108744357942183E-4	0.0	0.9739729240039303	4.502986123083336E-4
50-51	0.0	1.0108744357942183E-4	0.0	1.155401910809997	4.502986123083336E-4
52-53	0.0	1.0108744357942183E-4	0.0	1.3313262268247845	4.778679151027214E-4
54-55	0.0	1.0108744357942183E-4	0.0	1.5258598222257838	4.778679151027214E-4
56-57	0.0	1.0108744357942183E-4	0.0	1.7487897995050024	4.8705768270085065E-4
58-59	0.0	1.0108744357942183E-4	0.0	1.999165017716034	4.962474502989799E-4
60-61	0.0	1.1487209497661572E-4	0.0	2.2582015918883025	5.146269854952384E-4
62-63	0.0	1.1946697877568035E-4	0.0	2.520950832170215	5.513860558877554E-4
64-65	0.0	1.1946697877568035E-4	0.0	2.788451182300361	5.605758234858847E-4
66-67	4.594883799064629E-6	1.1946697877568035E-4	0.0	3.089246060438528	5.835502424812078E-4
68-69	9.189767598129257E-6	1.1946697877568035E-4	0.0	3.423151671232756	5.973348938784018E-4
70-71	9.189767598129257E-6	1.286567463738096E-4	0.0	3.783261904333049	6.157144290746602E-4
72-73	9.189767598129257E-6	1.3784651397193886E-4	0.0	4.146712617955262	6.662581508643712E-4
74-75	9.189767598129257E-6	1.3784651397193886E-4	0.0	4.498887486734571	6.80042802261565E-4
76-77	9.189767598129257E-6	1.3784651397193886E-4	0.0	4.88441202212749	6.892325698596943E-4
78-79	9.189767598129257E-6	1.3784651397193886E-4	0.0	5.3081384214258325	6.892325698596943E-4
80-81	9.189767598129257E-6	1.3784651397193886E-4	0.0	5.75409486854405	7.076121050559528E-4
82-83	9.189767598129257E-6	1.4703628157006812E-4	0.0	6.212655081923103	8.041046648363101E-4
84-85	9.189767598129257E-6	1.5622604916819738E-4	0.0	6.66077571931068	8.178893162335038E-4
86-87	9.189767598129257E-6	1.5622604916819738E-4	0.0	7.127335625383902	8.500535028269563E-4
88	9.189767598129257E-6	1.5622604916819738E-4	0.0	7.505228058786575	8.730279218222795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18655	0.0	50.742188	1
GTATCAA	32855	0.0	39.132847	1
GTACTAG	7110	0.0	32.57084	1
CAACGCA	38140	0.0	32.50617	5
ATCAACG	38640	0.0	32.062393	3
TATCAAC	41800	0.0	30.55968	2
AACGCAG	42560	0.0	29.65979	6
TCAACGC	43335	0.0	28.956903	4
GTGGTAT	6875	0.0	27.647089	1
ACGCAGA	46460	0.0	26.563128	7
TAGGCAT	9180	0.0	26.058342	5
CGCAGAG	47585	0.0	25.875984	8
GTACATG	47960	0.0	25.755701	1
TAGTACT	10020	0.0	25.750416	4
CTGTGCG	10155	0.0	25.68495	9
TACATGG	48550	0.0	25.052479	2
CTAGGCA	9900	0.0	24.780779	4
ACATGGG	48100	0.0	24.54495	3
GCATCGT	8595	0.0	24.058718	8
CTAGTAC	11570	0.0	23.31665	3
>>END_MODULE