FastQCFastQC Report
Thu 26 May 2016
ERR580167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4759232
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95240.20011632128881296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71740.15073860656509286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.048.5651861
GTATCAA136850.043.060281
CAACGCA160650.035.9734735
ATCAACG164300.035.207373
TATCAAC169900.034.4355282
AACGCAG179150.032.5854876
TCAACGC179350.032.356844
ACGCAGA195850.029.350657
CGCAGAG203000.028.270278
GTACTAG26800.027.024431
GTGGTAT30750.026.4588891
GCAGAGT221150.025.2054429
GTACATG207800.023.265811
TACATGG209100.022.6043362
GAGTACT93150.021.2164612-13
ACATGGG214700.021.2035873
GCATCGT27650.020.9083758
TGGTATC36600.020.8167842
TACCCCG4400.020.3054285
CAGAGTA216800.020.29145410-11
Produced by FastQC (version 0.11.5)