FastQCFastQC Report
Thu 26 May 2016
ERR580167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4759232
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80590.16933404381211087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65280.13716498796444468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72750.048.0134161
GTATCAA133400.042.8533251
CAACGCA158950.034.537695
ATCAACG162100.033.9245263
TATCAAC170600.033.1992382
AACGCAG177750.031.7550226
TCAACGC175050.031.441684
ACGCAGA198600.027.7349537
GTGGTAT27700.027.6639771
CGCAGAG205350.026.6404698
GCAGAGT226300.023.8211359
GTACATG203900.023.7710441
TACATGG207250.022.9058782
TACCCCG4700.022.0006665
ACATGGG209200.021.79323
GTGATCG16350.020.984028
AGAGTAC206150.020.97432910-11
GGCTATT34500.020.8425757
GGGCTAT35750.020.7711266
GAGTACT97800.020.66394212-13
Produced by FastQC (version 0.11.5)