FastQCFastQC Report
Thu 26 May 2016
ERR580166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924420
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137360.23185391987738885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111130.18757954365153043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61220.10333501000941864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89750.052.7639961
GTATCAA165000.041.2408141
CAACGCA187100.035.522355
ATCAACG188850.035.1916963
TATCAAC199350.033.793212
AACGCAG204000.033.1413086
TCAACGC209450.032.001134
ACGCAGA223950.029.5816087
GTACTAG29850.028.9878581
CGCAGAG229950.028.6256088
GTGGTAT36050.025.5678061
GCAGAGT254500.025.2947569
GTACATG217050.024.6561431
TACATGG220100.023.943362
ACATGGG219450.022.8740713
GCATCGT33850.022.4856998
GAGTACT134650.022.38801412-13
CAGAGTA253250.021.40410210-11
CATGGGG172600.021.3229394
TAGGCAT43100.021.156835
Produced by FastQC (version 0.11.5)