FastQCFastQC Report
Thu 26 May 2016
ERR580166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924420
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122060.2060286070197589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106540.17983194979424147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84700.051.1781921
GTATCAA154650.041.8013421
CAACGCA182050.034.620735
ATCAACG183850.034.2823523
TATCAAC194950.033.0872762
AACGCAG202800.032.0038226
TCAACGC201900.031.5428624
ACGCAGA222500.028.2197137
CGCAGAG228650.027.296028
GTACTAG30100.025.3038121
GTACATG213400.024.8955361
GTGGTAT30450.024.704161
TACATGG215200.024.2717952
GCAGAGT253500.024.0265929
ACATGGG214500.023.051223
GAGTACT134000.021.9207312-13
CATGGGG167350.021.2602464
TAGTACT40600.020.6059254
AGAGTAC234600.020.3732310-11
CAGAGTA249750.020.32287810-11
Produced by FastQC (version 0.11.5)