FastQCFastQC Report
Thu 26 May 2016
ERR580165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7173354
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179670.25046860924471315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143730.20036652310760072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.1211148926987292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106400.046.7653961
GTATCAA187100.040.1431731
GTACTAG53250.035.3281561
CAACGCA210450.034.1885455
ATCAACG216600.033.3305663
TATCAAC236400.031.5142142
AACGCAG240400.030.8713446
GCATCGT55350.029.7198188
TCAACGC246300.029.5772974
TAGGCAT67150.028.0825465
GTACATG270950.027.6854841
ACGCAGA260300.027.6625657
CTAGGCA71600.027.6528934
TAGTACT72800.027.3908794
TACATGG274500.026.9173682
CTGTGCG75000.026.6956839
CGCAGAG269350.026.6451268
CTAACGC24100.026.5408333
GGGCACC68700.025.99714394
GTGGTAT35750.025.9161661
Produced by FastQC (version 0.11.5)