FastQCFastQC Report
Thu 26 May 2016
ERR580165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7173354
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156740.2185030879557875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131940.18393069685394028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84680.11804798703646859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108800.044.54981
GTATCAA182950.038.0316621
GTACTAG51150.032.7205351
CAACGCA208300.031.702325
ATCAACG213200.030.930263
TATCAAC233800.029.4926532
GTACATG265900.028.8724671
AACGCAG239500.028.6115176
TAGTACT75550.028.3683724
TACATGG273100.027.916272
GGGCACC69050.027.63967994
CTGTGCG78750.027.4530189
TCAACGC244700.027.2553924
ACATGGG272100.026.8087313
CTAGTAC84050.026.7302673
TAGGCAT68650.026.6325685
ACGCAGA258350.025.7049357
CTAGGCA73700.025.5091824
GTACTGT91100.025.1246196
CATGGGG214100.024.9382324
Produced by FastQC (version 0.11.5)