FastQCFastQC Report
Thu 26 May 2016
ERR580164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7502968
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206650.27542433874168193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172290.22962912809970665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101280.13498658131022284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121000.050.0141031
GTATCAA217950.039.848261
CAACGCA242450.034.080635
ATCAACG246400.033.514543
TATCAAC273300.031.4433382
GTACTAG63450.031.0514371
AACGCAG275750.030.8523246
GTGGTAT36800.030.5386181
GCATCGT60750.029.6175428
TCAACGC287100.029.0920184
ACGCAGA287150.028.6301027
CGCAGAG290400.028.2128168
GGGCACC62050.027.72733994
TAGGCAT77250.027.4403065
GTACATG273050.027.0196721
CGTTAAC13750.026.674191
TACATGG275000.026.2915212
CTGTGCG74900.026.2854659
ACATGGG272400.025.5879963
GCAGAGT318300.025.1840659
Produced by FastQC (version 0.11.5)