FastQCFastQC Report
Thu 26 May 2016
ERR580164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7502968
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179230.23887880102913941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159870.2130756788513559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94830.12638998327062037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121200.047.8333171
GTATCAA219050.039.9889141
CAACGCA247150.033.8130875
ATCAACG250800.033.3584753
TATCAAC277750.031.351612
GGGCACC62900.031.30643794
AACGCAG284900.030.4674456
GTACTAG66300.029.2891351
TCAACGC294000.028.824524
CTGTGCG75500.028.8193429
GTGGTAT41000.027.9818211
ACGCAGA298250.027.9691647
CGCAGAG301150.027.4813528
TACCCCG4750.026.7167645
TAGGCAT77850.026.5648735
TAGTACT87000.025.9319784
GTACATG276450.024.7976761
GCAGAGT331750.024.6200479
ACTGTGC97750.024.4716138
TACATGG277400.024.238322
Produced by FastQC (version 0.11.5)