FastQCFastQC Report
Thu 26 May 2016
ERR580163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9063996
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205130.22631298601632216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156440.17259495701454414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100100.11043694194039803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123550.045.222731
GTATCAA216100.041.6335331
CAACGCA248000.035.0595175
ATCAACG256300.033.8930743
TATCAAC274550.032.58322
AACGCAG279650.031.8672926
TCAACGC283150.030.8270874
GTACTAG53300.030.7068371
GTACATG324400.028.4881841
ACGCAGA308150.028.2024487
TACATGG333150.027.3601592
CGCAGAG319750.027.1043198
ACATGGG336700.026.1629873
GCATCGT57700.024.924928
CATGGGG273900.024.6737864
TAGTACT77700.024.4529574
GTGGTAT45050.024.2201161
CTGTGCG80100.023.7637759
TAGGCAT72900.023.737835
GCAGAGT354600.023.6985479
Produced by FastQC (version 0.11.5)