FastQCFastQC Report
Thu 26 May 2016
ERR580163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9063996
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183330.20226178387545626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146030.16110995635920403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94870.10466685995889671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123100.045.3667761
GTATCAA211550.039.8869631
CAACGCA247800.032.793265
ATCAACG253700.032.03083
GTACTAG51950.031.1280861
TATCAAC277900.030.3585592
AACGCAG279800.029.68016
TCAACGC282850.028.9622364
GTACATG314350.027.9495871
TACATGG319900.027.1661382
CTAACGC24000.026.241393
ACGCAGA309850.026.240447
GTGGTAT47900.026.0069771
ACATGGG321900.025.8723583
TCTAACG25150.025.6028212
CGCAGAG321900.025.2290958
TAACGCC25850.024.3632434
CATGGGG264200.023.9621434
CTGTGCG76300.022.8523379
ATGGGGG187750.022.804965
Produced by FastQC (version 0.11.5)