FastQCFastQC Report
Thu 26 May 2016
ERR580162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5599777
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159870.28549351161662334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130820.23361644579775231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79380.14175564491228845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.054.4206921
GTATCAA151600.041.3752561
ATCAACG187500.032.635623
CAACGCA186950.032.6325345
TATCAAC196100.031.7615682
TCAACGC200700.030.5605244
AACGCAG204500.030.2095186
GTACATG216450.028.4792881
TACATGG220100.027.6148152
ACGCAGA225250.027.1140127
CGCAGAG229600.026.5389258
ACATGGG220700.026.4272253
CATGGGG173900.024.702694
ATGGGGG128650.024.2948045
GTGGTAT32450.024.1983531
GCAGAGT253600.023.6600199
GTACTAG26800.022.4573861
TGGGGGG122800.021.997886
GAGTACT148200.021.13428112-13
GCATCGT23950.020.5967668
Produced by FastQC (version 0.11.5)