FastQCFastQC Report
Thu 26 May 2016
ERR580162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5599777
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144490.2580281321916926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129740.23168779756765315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76640.13686259292111097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86400.052.5598031
GTATCAA156650.040.783021
CAACGCA189500.032.888145
ATCAACG189850.032.8033373
TATCAAC201550.031.6057592
TCAACGC202250.030.8845624
AACGCAG206400.030.7626346
GTACATG208950.027.8077871
ACGCAGA225550.027.5048317
GTACTAG27600.027.4225041
TACATGG212200.026.7849392
CGCAGAG232300.026.5835118
GTGGTAT36350.026.1238571
ACATGGG213600.025.877293
CATGGGG164450.024.6364634
GCAGAGT255850.023.804699
ATGGGGG123000.023.5003875
TGGGGGG111700.023.0992036
TGGTATC39800.022.7973292
TACCTGG31800.022.1755222
Produced by FastQC (version 0.11.5)