FastQCFastQC Report
Thu 26 May 2016
ERR580161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8434970
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200150.23728596545097375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160060.18975763992047393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88810.10528786705821123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141900.047.780661
GTATCAA256150.040.9335861
CAACGCA285800.034.9621055
ATCAACG292050.034.1974033
TATCAAC320150.032.4202842
AACGCAG325850.031.4015526
GTACTAG67650.030.6506771
GTGGTAT46600.030.3703541
ACGCAGA342000.029.013117
TCAACGC350050.028.8007684
CGCAGAG345600.028.616078
GTACATG402300.027.5355991
TAGGCAT85000.027.3705045
CTGTGCG86700.026.881029
TACATGG411600.026.5875852
ACATGGG400800.026.3960653
GGGCACC76900.026.34583394
TAGTACT89500.025.9949534
GCAGAGT376600.025.9517219
GCATCGT75600.025.1689368
Produced by FastQC (version 0.11.5)