FastQCFastQC Report
Thu 26 May 2016
ERR580161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8434970
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176660.20943761507154143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146250.17338532324359188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147900.048.379491
GTATCAA256850.039.059771
CAACGCA285950.033.729065
ATCAACG294150.032.85333
TATCAAC323300.030.9010492
GTACTAG66700.030.096561
AACGCAG331750.030.0464156
GTGGTAT47200.029.9805321
TCAACGC352350.027.866914
TAGGCAT83950.027.3782165
ACGCAGA349750.027.3444757
CGCAGAG353100.026.9917188
GGGCACC76300.026.9181794
GTACATG388800.026.2874321
TACATGG395400.025.5754782
TACCCCG6300.025.3661945
ACATGGG391200.025.0994553
CTGTGCG90250.024.8379679
CTAGGCA94600.024.4951734
TAGTACT93800.024.25314
Produced by FastQC (version 0.11.5)