FastQCFastQC Report
Thu 26 May 2016
ERR580160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578671
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89250.19492555809316722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66750.14578466109488977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.045.2458951
GTATCAA134900.041.97221
CAACGCA152300.035.4751745
ATCAACG159100.034.0217823
TATCAAC169400.033.036212
AACGCAG173400.032.009746
TCAACGC178800.030.5609464
GTACTAG34050.030.108321
GTGGTAT25000.029.532951
ACGCAGA189450.028.5057
CGCAGAG194650.027.6946018
CGTTAAC9950.025.5221841
GCATCGT34400.025.4121028
GCAGAGT212950.024.8299129
TAGGCAT43450.024.1334555
GTACATG215300.023.0875641
CTAGGCA47950.023.0466294
TACATGG217850.022.5155932
ACATGGG218850.021.7033483
GTGATCG22750.021.6917218
Produced by FastQC (version 0.11.5)