FastQCFastQC Report
Thu 26 May 2016
ERR580160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578671
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75750.16544101989420074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57780.12619382349157648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.046.768921
GTATCAA126500.042.6579251
CAACGCA151900.034.190135
ATCAACG154900.033.467273
TATCAAC163400.033.1076972
AACGCAG173000.030.9699676
TCAACGC173300.030.103754
ACGCAGA189850.027.4538277
CGCAGAG194550.026.8147478
GTACTAG32650.024.762451
CGTTAAC10250.024.3052671
GCAGAGT213400.023.8955639
GTGGTAT24750.023.7401351
TACCCCG5050.023.2672395
GTACATG208100.022.836391
TATTCTC23700.022.6075085
TACATGG211200.022.098412
GGGCTAT41400.021.7963036
GCTATTG40400.021.6378238
ACATGGG209950.021.4459763
Produced by FastQC (version 0.11.5)