FastQCFastQC Report
Thu 26 May 2016
ERR580159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107087
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36930.11885730911300521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.10447084359079743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.047.86661
GTATCAA85800.038.753241
CAACGCA97350.032.8478435
ATCAACG98300.032.4872633
TATCAAC105650.031.3840872
AACGCAG108250.030.0036546
TCAACGC110500.029.2399984
GTGGTAT17900.027.062011
ACGCAGA119550.026.6561367
CGCAGAG124550.025.5474788
GTACATG143650.023.2776891
GTACTAG16800.023.2351061
GCAGAGT135800.022.5665369
TACATGG145000.022.4778672
ACATGGG148750.021.3108023
TAGTACT27500.021.0356184
CTGTGCG27100.020.6392889
GAGTACT45600.020.35627612-13
CATGGGG122600.019.832754
CTAGTAC29950.019.6295363
Produced by FastQC (version 0.11.5)