FastQCFastQC Report
Thu 26 May 2016
ERR580159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107087
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31640.10183171568739464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46900.042.396841
GTATCAA78950.039.475481
CAACGCA89150.033.213555
ATCAACG91400.032.6016163
TATCAAC98400.031.1914182
AACGCAG101200.030.0468446
TCAACGC103000.029.021244
ACGCAGA113900.026.1601757
CGCAGAG118900.025.0205578
GTACTAG19100.024.85731
TACCTGG20200.023.7337232
GTGGTAT18350.023.5677281
CGTTAAC6800.023.5036871
GTACATG135950.022.7862151
TACATGG137200.022.2677462
GCAGAGT130950.022.2516029
TAGGCAT25150.022.0515985
ACATGGG139950.021.2246363
GTACCTG23500.020.8032631
TAGTACT25400.019.9841694
Produced by FastQC (version 0.11.5)