FastQCFastQC Report
Thu 26 May 2016
ERR580158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064523
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331300.2535875209527359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266480.203972238404724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149700.11458512492189725No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202850.048.0097541
GTATCAA363900.040.4599531
CAACGCA417000.033.8819165
ATCAACG425000.033.2250633
TATCAAC454450.032.2015082
AACGCAG465500.030.8814776
GTACTAG86150.029.7485331
TCAACGC480300.029.7125914
GTGGTAT75750.029.5494041
ACGCAGA498250.028.096677
CGCAGAG509600.027.3780468
GCATCGT89900.025.2469588
TAGGCAT109900.025.198645
CTAACGC40300.024.96943
GTACATG486150.024.704441
GCAGAGT559650.024.3123479
TCTAACG42800.023.951652
TACATGG490700.023.8332042
TAACGCC42600.023.2898834
CTGTGCG110700.023.2663219
Produced by FastQC (version 0.11.5)